ACE Antibody (3H536): sc-69992

ACE Antibody (3H536) is a mouse monoclonal IgG1 kappa light chain antibody engineered to target angiotensin-converting enzyme (ACE), also known as CD143 or Angiotensin Converting Enzyme 1. Anti-ACE antibody (3H536) is raised against human kidney-derived ACE and effectively detects ACE proteins from mouse, rat, and human origins through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). ACE monoclonal antibody (3H536) is available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, providing versatile options for diverse research needs. Angiotensin-converting enzyme (ACE) is a membrane-bound zinc metallopeptidase that plays a pivotal role in the renin-angiotensin system by converting Angiotensin I into the potent vasopressor hormone Angiotensin II, thereby regulating blood pressure and electrolyte balance. ACE undergoes essential post-translational modifications, including N-linked glycosylation, which are critical for proper folding, stability, and enzymatic activity. These glycosylation sites enhance ACE′s interaction with substrates and inhibitors, making post-translational modifications vital for biological function. Two primary isoforms of ACE exist: pulmonary ACE (ACEP), a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney, and spleen, and testicular ACE (ACET), which is exclusively expressed in adult testis by developing sperm cells, specifically late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, contributing to fluid and electrolyte homeostasis. Understanding the significance of ACE′s post-translational modifications is essential for researching cardiovascular diseases, hypertension, and related disorders, making anti-ACE antibody (3H536) an indispensable tool for biomedical research.

The preferred ACE antibody is ACE Antibody (2E2): sc-23908.