ZC3H14 Double Nickase Plasmid (m): sc-429118-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZC3H14 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZC3H14 Double Nickase Plasmid (m) and ZC3H14 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Zc3h14. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZC3H14 Double Nickase Plasmid (m) | sc-429118-NIC | 20 µg | $410.00 |
Mouse Zc3h14 encodes ZC3H14, a CCCH-type zinc finger RNA-binding protein that associates with poly(A) RNA and regulates post-transcriptional gene control through effects on mRNA polyadenylation, stability, and translation. ZC3H14 contributes to RNA processing programs that shape gene expression during neuronal development and synaptic function, linking it to broader mRNA metabolism pathways in the nucleus and cytoplasm. Disruption of ZC3H14 orthologs has been connected to neurodevelopmental phenotypes and altered neuronal gene expression, making this factor relevant for mechanistic studies of RNA regulation in brain-relevant cell types. As a conserved regulator of mRNA fate, ZC3H14 is frequently interrogated in models of neuronal differentiation, RNA granule dynamics, and transcriptome-wide poly(A) tail homeostasis.
ZC3H14 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Zc3h14 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Zc3h14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Zc3h14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Zc3h14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.