Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
YY2 Double Nickase Plasmid (m): sc-437263-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- YY2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- YY2 Double Nickase Plasmid (m) and YY2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Yy2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: YY2 Antibody (A-5): sc-377008
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
YY2 Double Nickase Plasmid (m) | sc-437263-NIC | 20 µg | $410.00 |
Mouse Yy2 encodes YY2, a C2H2 zinc-finger transcription factor related to YY1 that binds GC-rich DNA elements and modulates promoter activity to shape gene-expression programs. YY2 has been implicated in transcriptional control linked to chromatin organization, developmental regulation, and cell-cycle–associated gene networks, where it can act as a context-dependent activator or repressor. Through these regulatory functions, Yy2 provides a handle for studying transcriptional circuitry and genome-wide binding dynamics that influence cellular identity and stress responses. Dysregulation of YY-family transcriptional programs is relevant to models of aberrant proliferation and differentiation, supporting Yy2 as a research target in mechanistic studies of gene regulation.
YY2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Yy2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Yy2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Yy2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Yy2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.