Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
XPA Double Nickase Plasmid (h): sc-401483-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- XPA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- XPA Double Nickase Plasmid (h) and XPA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting XPA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: XPA Antibody (B-1): sc-28353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
XPA Double Nickase Plasmid (h) | sc-401483-NIC | 20 µg | $410.00 | |||
XPA Double Nickase Plasmid (h2) | sc-401483-NIC-2 | 20 µg | $410.00 |
XPA (xeroderma pigmentosum group A) encodes a core DNA damage recognition and verification factor in nucleotide excision repair (NER), where it stabilizes repair intermediates and coordinates recruitment of endonucleases and replication protein A at sites of bulky helix-distorting lesions. The XPA protein is critical for removing UV-induced photoproducts and chemical adducts, thereby preserving genome integrity and limiting mutagenesis during replication and transcription. Disruption of XPA function is linked to the DNA repair–deficient disorder xeroderma pigmentosum, and XPA status is widely used to probe NER capacity, replication stress responses, and DNA damage signaling crosstalk.
XPA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the XPA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within XPA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt XPA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of XPA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.