VEZF1 CRISPR/Cas9 KO Plasmid (m): sc-423670
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VEZF1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VEZF1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VEZF1 CRISPR/Cas9 KO Plasmid (m) | sc-423670 | 20 µg | $397.00 |
Overview
Vezf1 encodes VEZF1, a zinc finger transcription factor enriched in vascular endothelial and hematopoietic contexts that regulates gene expression programs required for embryonic angiogenesis and vascular patterning. VEZF1 functions in the control of endothelial differentiation, vessel sprouting, and maintenance of vascular integrity by coordinating transcriptional networks linked to cell migration, extracellular matrix remodeling, and developmental signaling pathways. Altered Vezf1 activity has been associated with defects in blood vessel formation and developmental lethality in mouse models, and its regulatory roles make it relevant to studying vascular malformations and impaired tissue vascularization. As a nuclear DNA-binding protein, VEZF1 also provides a tractable node for dissecting transcriptional control of endothelial lineage specification.
VEZF1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Vezf1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Vezf1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Vezf1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VEZF1 protein expression.
This CRISPR knockout system enables efficient generation of Vezf1-deficient cell models for investigation of VEZF1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Vezf1 exon(s) critical for VEZF1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Vezf1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VEZF1 CRISPR/Cas9 KO Plasmid (m) and VEZF1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Vezf1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VEZF1 HDR Plasmid (m) and VEZF1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Vezf1 homology arms to support homology-directed repair at defined Vezf1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.