Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
UMPS CRISPR/Cas9 KO Plasmid (m): sc-423609
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UMPS CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UMPS genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: UMPS Antibody (A-9): sc-398086
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UMPS CRISPR/Cas9 KO Plasmid (m) | sc-423609 | 20 µg | $397.00 |
Overview
Umps encodes uridine monophosphate synthetase (UMPS), a bifunctional enzyme that catalyzes the terminal steps of de novo pyrimidine biosynthesis, converting orotate to UMP via orotate phosphoribosyltransferase and orotidine-5′-phosphate decarboxylase activities. UMPS supports nucleotide pool homeostasis required for DNA replication, RNA synthesis, and cell-cycle progression, linking pyrimidine metabolism to proliferative capacity and genome stability. Perturbation of UMPS function impacts one-carbon and folate-coupled metabolic networks indirectly through altered nucleotide demand and can reshape cellular responses to replication stress. Dysregulation of pyrimidine synthesis pathways is broadly relevant to studies of metabolic disease mechanisms and proliferative phenotypes in mammalian systems, including mouse models.
UMPS CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Umps gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Umps together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Umps open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UMPS protein expression.
This CRISPR knockout system enables efficient generation of Umps-deficient cell models for investigation of UMPS signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Umps exon(s) critical for UMPS function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Umps genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UMPS CRISPR/Cas9 KO Plasmid (m) and UMPS CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Umps locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UMPS HDR Plasmid (m) and UMPS HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Umps homology arms to support homology-directed repair at defined Umps target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.