TSLP Antibody (LX-9): sc-80410

TSLP Antibody (LX-9) is a rat monoclonal IgG2a antibody that detects TSLP protein of mouse and rat origin by western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). Anti-TSLP antibody (LX-9) is available as the non-conjugated form. Thymic stromal lymphopoietin (TSLP) plays a crucial role in the immune system as a cytokine that promotes the development and differentiation of B cells, which are essential for adaptive immunity. The gene encoding murine TSLP is located on chromosome 18, and human TSLP is expressed in various tissues, including the heart, liver, and prostate, highlighting TSLP′s importance in both species. TSLP exerts biological effects by binding to a specific receptor complex; TSLP first interacts with the TSLP receptor (TSLPR) with low affinity, subsequently forming a high-affinity complex with the interleukin-7 receptor alpha (IL-7Ra) subunit. This interaction elucidates the overlapping functions of TSLP and interleukin-7, particularly in the activation of the transcription factor Stat5, which is critical for cell survival and proliferation. While both TSLP and IL-7 induce Stat5 phosphorylation, TSLP signaling does not activate Janus kinases (JAKs), distinguishing TSLP′s pathway from IL-7. TSLP prevents apoptosis and stimulates the proliferation of myeloid cells, a function supported by the coexpression of TSLPR and IL-7Ra on monocytes and dendritic cells, underscoring TSLP′s vital role in immune responses and potential implications in various diseases.

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TSLP Antibody (LX-9) References:

1. Requirement for stat5 in thymic stromal lymphopoietin-mediated signal transduction.  |  Isaksen, DE., et al. 1999. J Immunol. 163: 5971-7. PMID: 10570284
2. Cloning of a receptor subunit required for signaling by thymic stromal lymphopoietin.  |  Pandey, A., et al. 2000. Nat Immunol. 1: 59-64. PMID: 10881176
3. Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor.  |  Park, LS., et al. 2000. J Exp Med. 192: 659-70. PMID: 10974032
4. Molecular cloning and biological characterization of a novel murine lymphoid growth factor.  |  Sims, JE., et al. 2000. J Exp Med. 192: 671-80. PMID: 10974033
5. Human thymic stromal lymphopoietin preferentially stimulates myeloid cells.  |  Reche, PA., et al. 2001. J Immunol. 167: 336-43. PMID: 11418668
6. Cloning of human thymic stromal lymphopoietin (TSLP) and signaling mechanisms leading to proliferation.  |  Quentmeier, H., et al. 2001. Leukemia. 15: 1286-92. PMID: 11480573
7. Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma.  |  Verstraete, K., et al. 2017. Nat Commun. 8: 14937. PMID: 28368013
8. TSLP and IL-33 reciprocally promote each other's lung protein expression and ILC2 receptor expression to enhance innate type-2 airway inflammation.  |  Toki, S., et al. 2020. Allergy. 75: 1606-1617. PMID: 31975538
9. Role of IL-25, IL-33, and TSLP in triggering united airway diseases toward type 2 inflammation.  |  Hong, H., et al. 2020. Allergy. 75: 2794-2804. PMID: 32737888
10. Thymic Stromal Lymphopoietin (TSLP), Its Isoforms and the Interplay with the Epithelium in Allergy and Asthma.  |  Smolinska, S., et al. 2023. Int J Mol Sci. 24: PMID: 37628907
11. Skin microbe-dependent TSLP-ILC2 priming axis in early life is co-opted in allergic inflammation.  |  Cha, J., et al. 2024. Cell Host Microbe. 32: 244-260.e11. PMID: 38198924
12. Thymic stromal lymphopoietin: a cytokine that promotes the development of IgM+ B cells in vitro and signals via a novel mechanism.  |  Levin, SD., et al. 1999. J Immunol. 162: 677-83. PMID: 9916685