TRPC5 Double Nickase Plasmid (h): sc-403745-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TRPC5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TRPC5 Double Nickase Plasmid (h) and TRPC5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TRPC5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TRPC5 Antibody (1C8): sc-293259
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TRPC5 Double Nickase Plasmid (h) | sc-403745-NIC | 20 µg | $410.00 | |||
TRPC5 Double Nickase Plasmid (h2) | sc-403745-NIC-2 | 20 µg | $410.00 |
TRPC5 (transient receptor potential cation channel subfamily C member 5) encodes a Ca2+-permeable, nonselective cation channel that functions as a sensor of extracellular and intracellular cues to regulate membrane excitability and calcium-dependent signaling. In human cells, TRPC5 contributes to receptor-operated calcium entry downstream of GPCR and receptor tyrosine kinase pathways, intersecting with PLC signaling, phosphoinositide metabolism, and downstream transcriptional programs such as NFAT and MAPK. TRPC5 activity influences cytoskeletal dynamics, cell migration, and barrier function through Ca2+-dependent effectors, linking channel gating to mechanochemical responses. Dysregulated TRPC5 signaling has been associated with contexts relevant to neurobiology, renal and cardiovascular physiology, and cellular stress responses, making it a useful target for pathway dissection in disease-relevant models.
TRPC5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TRPC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TRPC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TRPC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TRPC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.