Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
tropomodulin 3 CRISPR/Cas9 KO Plasmid (h): sc-405617
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- tropomodulin 3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the tropomodulin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: tropomodulin 3 Antibody (F-11): sc-365646
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
tropomodulin 3 CRISPR/Cas9 KO Plasmid (h) | sc-405617 | 20 µg | $397.00 |
Overview
TMOD3 encodes tropomodulin 3, an actin filament pointed-end capping protein that regulates filament length and stability, supporting cortical actin organization, cell shape, and mechanical properties. By modulating actin dynamics, TMOD3 contributes to cytoskeletal remodeling processes such as cell migration, adhesion, and cytokinesis, and interfaces functionally with pathways governing membrane trafficking and actomyosin contractility. Altered actin regulation has been implicated across diverse disease contexts where cytoskeletal organization is disrupted, including cancer cell invasion and hematologic cell dysfunction, making TMOD3 a useful node for studying cytoskeleton-dependent phenotypes. TMOD3 is also relevant for investigating erythroid and immune cell architecture where precise actin filament control is required for normal cellular function.
tropomodulin 3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMOD3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMOD3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMOD3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish tropomodulin 3 protein expression.
This CRISPR knockout system enables efficient generation of TMOD3-deficient cell models for investigation of tropomodulin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMOD3 exon(s) critical for tropomodulin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMOD3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by tropomodulin 3 CRISPR/Cas9 KO Plasmid (h) and tropomodulin 3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMOD3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by tropomodulin 3 HDR Plasmid (h) and tropomodulin 3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMOD3 homology arms to support homology-directed repair at defined TMOD3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.