Tris-EDTA buffer solution

Tris-EDTA (TE) buffer solution is a fundamental reagent extensively employed in molecular biology research, particularly in nucleic acid manipulation and storage. This buffer is meticulously formulated to provide stable and physiologically relevant conditions for the maintenance, manipulation, and analysis of nucleic acids. In research, TE buffer solution finds diverse applications, including nucleic acid extraction, purification, storage, and molecular biology techniques such as polymerase chain reaction (PCR), restriction enzyme digestion, and DNA sequencing. It is commonly used to resuspend or dilute DNA or RNA samples, providing a stable environment that helps prevent nucleic acid degradation and maintains their integrity during experimental procedures. Moreover, TE buffer is essential for nucleic acid storage, as it helps protect DNA and RNA from enzymatic degradation and chemical damage. It is often used to resuspend DNA pellets or to elute nucleic acids from purification columns before storage at low temperatures, such as -20°C or -80°C. Ongoing research efforts focus on optimizing TE buffer formulations and exploring its applications in emerging nucleic acid technologies, such as next-generation sequencing and single-cell genomics.