Prior to use, dilute to a 1X solution with ddH20. Filter solution through a 0.22 μm filter into a sterile flask. Makes 5 L of 1X TBS buffer. Store the buffer solution at +4°C. Final (1X) pH 7.4 ±0.2 (25°C), may need slight pH adjustment.
pH :
When diluted to a 1X concentration, the pH will be ~7.4.