Date published: 2026-3-19

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trans-3′-Hydroxycotinine (CAS 34834-67-8)

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Application:
trans-3′-Hydroxycotinine is a metabolite of nicotine and a carcinogen
CAS Number:
34834-67-8
Molecular Weight:
192.22
Molecular Formula:
C10H12N2O2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Trans-3′-Hydroxycotinine is involved in the metabolism of nicotine. It is formed through the oxidation of nicotine by the enzyme cytochrome P450 2A6. Trans-3′-Hydroxycotinine plays a role in the detoxification and elimination of nicotine. Trans-3′-Hydroxycotinine is a major metabolite of nicotine, and its formation represents an important step in the breakdown of nicotine. It is produced in the liver and subsequently excreted in the urine. The presence of trans-3′-Hydroxycotinine in biological samples is indicative of recent nicotine exposure. Its formation and subsequent elimination contribute to the overall process of nicotine metabolism. At the molecular level, trans-3′-Hydroxycotinine interacts with specific enzymes and pathways involved in nicotine metabolism, influencing the overall disposition of nicotine.


trans-3′-Hydroxycotinine (CAS 34834-67-8) References

  1. Serum Concentrations of Cotinine and Trans-3'-Hydroxycotinine in US Adults: Results From Wave 1 (2013-2014) of the Population Assessment of Tobacco and Health Study.  |  Sosnoff, CS., et al. 2022. Nicotine Tob Res. 24: 736-744. PMID: 34897512
  2. Application of HPLC-QQQ-MS/MS and New RP-HPLC-DAD System Utilizing the Chaotropic Effect for Determination of Nicotine and Its Major Metabolites Cotinine, and trans-3'-Hydroxycotinine in Human Plasma Samples.  |  Baj, J., et al. 2022. Molecules. 27: PMID: 35163947
  3. Nicotine Exposure in the U.S. Population: Total Urinary Nicotine Biomarkers in NHANES 2015-2016.  |  Mazumder, S., et al. 2022. Int J Environ Res Public Health. 19: PMID: 35329347
  4. Effects of excessive alcohol drinking on nicotine biotransformation in rats.  |  Kasprzyk, J., et al. 2022. Sci Rep. 12: 11066. PMID: 35773467
  5. Simultaneous quantification of urinary tobacco and marijuana metabolites using solid-supported liquid-liquid extraction coupled with liquid chromatography tandem mass spectrometry.  |  Yakimavets, V., et al. 2022. J Chromatogr B Analyt Technol Biomed Life Sci. 1208: 123378. PMID: 35908438
  6. Effects of Genetic Variants in the Nicotine Metabolism Pathway on Smoking Cessation.  |  Li, H., et al. 2022. Genet Res (Camb). 2022: 2917881. PMID: 36245555
  7. Impact of Genetic Variants in the Nicotine Metabolism Pathway on Nicotine Metabolite Levels in Smokers.  |  Perez-Paramo, YX., et al. 2023. Cancer Epidemiol Biomarkers Prev. 32: 54-65. PMID: 36252563
  8. Relating individual differences in the reinforcing value of smoking and dependence severity to nicotine exposure levels in vulnerable populations.  |  Browning, KO., et al. 2022. Prev Med. 165: 107312. PMID: 36272516
  9. The two-tales of smoking: aberrations in sperm parameters and failure in assisted reproduction.  |  Arshad, MA., et al. 2022. Arab J Urol. 20: 195-196. PMID: 36353471
  10. Biomarker selection strategies based on compound stability in wastewater-based epidemiology.  |  Gao, Z., et al. 2023. Environ Sci Pollut Res Int. 30: 5516-5529. PMID: 36418835
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  13. Assessment of exposure and DNA damage from second-hand smoke using potential biomarker in urine: cigarettes and heated tobacco products.  |  Kawasaki, Y., et al. 2023. J Clin Biochem Nutr. 72: 242-247. PMID: 37251967

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

trans-3′-Hydroxycotinine, 10 mg

sc-220310
10 mg
$640.00