Date published: 2026-1-13

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Toluidine Blue (CAS 6586-04-5)

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Alternate Names:
Blutene chloride
Application:
Toluidine Blue is CI: 52040
CAS Number:
6586-04-5
Molecular Weight:
373.97
Molecular Formula:
C15H16ClN3S•0.5ZnCl2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Toluidine Blue is widely utilized in biological research, particularly in histology and microscopy, where it serves as a vital staining agent for differentiating between different cellular components and tissues. This chemical binds to acidic tissue components, causing them to appear distinct under a microscope, which helps in the detailed study of cell morphology and tissue structure. Researchers also employ Toluidine Blue in the field of biotechnology for staining nucleic acids, thereby aiding in the visualization of cellular RNA and DNA. This staining capacity is particularly valuable in various genomic studies, where understanding the interaction and location of nucleic acids is necessary. Additionally, Toluidine Blue is used in environmental science to detect the presence of fungal contaminants in various samples, assisting researchers in studying ecosystem impacts and microbial distribution.


Toluidine Blue (CAS 6586-04-5) References

  1. Comparison of the methylene blue and toluidine blue photobactericidal efficacy against gram-positive and gram-negative microorganisms.  |  Usacheva, MN., et al. 2001. Lasers Surg Med. 29: 165-73. PMID: 11553906
  2. Toluidine blue: aggregation properties and distribution behavior in surfactin micelle solution.  |  Liu, J., et al. 2010. Colloids Surf B Biointerfaces. 75: 496-500. PMID: 19854627
  3. Toluidine blue: rapid and simple malaria parasite screening and species identification.  |  Awale, R., et al. 2017. Pan Afr Med J. 28: 27. PMID: 29138663
  4. Toluidine Blue with a Synergistic Effect in Morphological Assessment of Oral Cytosmears.  |  Ratna Kumari, TVN. and Ahmed Mujib, BR. 2018. J Cytol. 35: 8-14. PMID: 29403163
  5. Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology.  |  Ghnenis, AB., et al. 2018. J Vis Exp.. PMID: 30035773
  6. Photoexcited Toluidine Blue Inhibits Tau Aggregation in Alzheimer's Disease.  |  Dubey, T., et al. 2019. ACS Omega. 4: 18793-18802. PMID: 31737841
  7. Ultrafast Toluidine Blue Staining for Rapid On-Site Evaluation of Cytological Smears.  |  Hewer, E. and Schmitt, AM. 2020. Acta Cytol. 64: 375-377. PMID: 31962321
  8. Evaluation of Toluidine Blue-Mediated Photodynamic Therapy for Experimental Bacterial Keratitis in Rabbits.  |  Su, G., et al. 2020. Transl Vis Sci Technol. 9: 13. PMID: 32714639
  9. Toluidine blue versus frozen section for assessment of mucosal tumor margins in oral squamous cell carcinoma.  |  Algadi, HH., et al. 2020. BMC Cancer. 20: 1147. PMID: 33238944
  10. Comparing the Efficacy of Toluidine Blue, Methylene Blue and Curcumin in Photodynamic Therapy Against Enterococcus faecalis.  |  Mozayeni, MA., et al. 2020. J Lasers Med Sci. 11: S49-S54. PMID: 33995969
  11. Toluidine blue staining of murine mast cells and quantitation by a novel, automated image analysis method using whole slide skin images.  |  Raja, N., et al. 2021. J Histotechnol. 44: 190-195. PMID: 33998401
  12. Punicalagin attenuates osteoarthritis progression via regulating Foxo1/Prg4/HIF3α axis.  |  Liu, F., et al. 2021. Bone. 152: 116070. PMID: 34171516
  13. Toluidine blue for the detection of sentinel lymph nodes in patients with thyroid cancer.  |  Moskalenko, Y., et al. 2022. Contemp Oncol (Pozn). 26: 259-267. PMID: 36816393
  14. Acetic acid versus toluidine blue as screening tools for oral potentially malignant disorders.  |  Winton Kalluvelil, SR. and Narayanan, VS. 2022. Indian J Cancer.. PMID: 36861696
  15. Photo-Excited Toluidine Blue Disaggregates the Repeat Tau and Modulates End-Binding Protein EB1, Cytoskeletal Structure in Neuronal Cells.  |  Chinnathambi, S., et al. 2023. Cell Physiol Biochem. 57: 123-136. PMID: 37074918

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Toluidine Blue, 5 g

sc-253710
5 g
$67.00