Date published: 2025-9-5
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
TLR2 Antibody (TL2.1) - Flow Cytometry - Image 5957
TLR2 抗体 (TL2. 1) FITC: sc-21759 FITC. THP-1 细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2a: sc-2856.
TLR2 Antibody (TL2.1) - Flow Cytometry - Image 5957
TLR2 抗体 (TL2. 1) FITC: sc-21759 FITC. THP-1 细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2a: sc-2856.
TLR2 Antibody (TL2.1) - Flow Cytometry - Image 5956
TLR2 抗体 (TL2. 1) PE: sc-21759 PE. THP-1 细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2a: sc-2867.
TLR2 Antibody (TL2.1) - Western Blotting - Image 32738
TLR2 抗体 (TL2. 1): sc-21759. THP-1 全细胞裂解液中 TLR2 表达的 Western 印迹分析.
TLR2 抗体 (TL2. 1) FITC: sc-21759 FITC. THP-1 细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG2a: sc-2856.

TLR2 抗体 (TL2.1): sc-21759

3.6(21)
寫評論提問

说明书
  • TLR2 抗体 TL2.1 是小鼠单克隆 IgG2a κ，TLR2抗体, 在59篇文献中引用，规格为200 µg/ml
  • 针对来源于human的CHO-TLR2细胞
  • TLR2 抗体 (TL2.1) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 TLR2
  • 抗TLR2抗体（TL2.1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联PerCP-Cy5.5 (sc-21759 PCPC5, 100 tests in 2 ml)，用于IF, IHC(P) 和 FCM
  • 关于如何获取TLR2 (TL2.1): sc-21759的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRP是TLR2 Antibody (TL2.1) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与TLR2 Antibody (TL2.1) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外TLR抗体，包括TLR1, TLR10, TLR2, TLR3, TLR4, TLR5, TLR7, TLR8和TLR9

TLR2抗体（TL2.1）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人源TLR2蛋白。TLR2 (TL2.1) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。TLR2 作为模式识别受体在免疫系统中起着至关重要的作用，可识别病原体相关分子模式，特别是来自革兰氏阳性细菌和某些病毒的模式。这一功能对于启动先天免疫反应至关重要，因为TLR2的激活会导致免疫细胞的募集和促炎细胞因子的产生，从而增强宿主抵抗感染的能力。TLR2主要位于免疫细胞（如巨噬细胞和树突状细胞）的细胞表面，在那里TLR2可以有效地检测细胞外的病原体。TLR2的结构包括一个高度保守的Toll/IL-1受体（TIR）结构域，该结构域对于下游信号传导至关重要，可激活NFκB等转录因子，从而协调炎症反应。TLR2在外周血白细胞、巨噬细胞和单核细胞中的表达尤为突出，这凸显了TLR2在免疫监控和感染反应中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

TLR2 抗体 (TL2.1) 参考文献:

  1. Toll 样受体-4 介导脂多糖诱导的信号转导。  |  Chow, JC., et al. 1999. J Biol Chem. 274: 10689-92. PMID: 10196138
  2. TLR6：不断扩大的收费样受体家族的新成员。  |  Takeuchi, O., et al. 1999. Gene. 231: 59-65. PMID: 10231569
  3. 肽聚糖和脂联素诱导的细胞活化是由收费样受体 2 介导的。  |  Schwandner, R., et al. 1999. J Biol Chem. 274: 17406-9. PMID: 10364168
  4. 微生物脂蛋白通过收费样受体触发的宿主防御机制。  |  Brightbill, HD., et al. 1999. Science. 285: 732-6. PMID: 10426995
  5. 果蝇 Toll 和 IL-1 受体  |  Gay, NJ. and Keith, FJ. 1991. Nature. 351: 355-6. PMID: 1851964
  6. TLR2激动剂及其结构活性关系。  |  Lu, BL., et al. 2020. Org Biomol Chem. 18: 5073-5094. PMID: 32582902
  7. TLR2 的硅氨酰化可启动破骨细胞融合。  |  Dou, C., et al. 2022. Bone Res. 10: 24. PMID: 35232979
  8. 双歧杆菌通过TLR2诱导 Decorin+巨噬细胞，抑制结肠癌的发生。  |  Lin, Y., et al. 2023. J Exp Clin Cancer Res. 42: 172. PMID: 37464382
  9. TLR2通过BMP信号传导调节毛囊周期和再生。  |  Xiong, L., et al. 2024. Elife. 12: PMID: 38483447
  10. 果蝇 Toll 蛋白的人类同源物发出了激活适应性免疫的信号。  |  Medzhitov, R., et al. 1997. Nature. 388: 394-7. PMID: 9237759
  11. 在结构上与果蝇 Toll 有关的人类受体家族。  |  Rock, FL., et al. 1998. Proc Natl Acad Sci U S A. 95: 588-93. PMID: 9435236
  12. Toll样受体-2介导脂多糖诱导的细胞信号。  |  Yang, RB., et al. 1998. Nature. 395: 284-8. PMID: 9751057

订购信息

产品名称产品编号规格价格数量收藏夹

TLR2 抗体 (TL2.1)

sc-21759
200 µg/ml
$316.00

TLR2 (TL2.1): m-IgG2a BP-HRP 套装

sc-545915
200 µg Ab; 10 µg BP
$354.00

TLR2 抗体 (TL2.1) AC

sc-21759 AC
500 µg/ml, 25% agarose
$416.00

TLR2 抗体 (TL2.1) HRP

sc-21759 HRP
200 µg/ml
$316.00

TLR2 抗体 (TL2.1) FITC

sc-21759 FITC
200 µg/ml
$330.00

TLR2 抗体 (TL2.1) PE

sc-21759 PE
200 µg/ml
$343.00

TLR2 抗体 (TL2.1) Alexa Fluor® 488

sc-21759 AF488
200 µg/ml
$357.00

TLR2 抗体 (TL2.1) Alexa Fluor® 546

sc-21759 AF546
200 µg/ml
$357.00

TLR2 抗体 (TL2.1) Alexa Fluor® 594

sc-21759 AF594
200 µg/ml
$357.00

TLR2 抗体 (TL2.1) Alexa Fluor® 647

sc-21759 AF647
200 µg/ml
$357.00

TLR2 抗体 (TL2.1) Alexa Fluor® 680

sc-21759 AF680
200 µg/ml
$357.00

TLR2 抗体 (TL2.1) Alexa Fluor® 790

sc-21759 AF790
200 µg/ml
$357.00

TLR2 抗体 (TL2.1) PerCP-Cy5.5

sc-21759 PCPC5
100 tests in 2 ml
$351.00

Is this compatible with Rhesus Macaque?

Asked by: Brad_Leishmania
Thank you for your question. This antibody has not yet been tested in Rhesus Macaque and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-02-18

Dears; I would like to ask what is the positive control tissue of Sc21759 HRP (TLR2) if will used IHC-P and what is recommended Dilution factor?

Asked by: shaikhah
Thank you for your question. We have not yet tested our TLR2 Antibody (TL2.1): sc-21759 for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, or IHC(P). However, we are aware of publications citing use of the antibody for this type of research application. For example, PMID's: 21564140 and 18607891. Our general protocol is provided below. We recommend using a starting dilution of 1:50, however if you are using the HRP conjugated primary antibody, it would be important to test for an optimal dilution with your samples. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-21

What is the best secondary antibody for detection in WB?

Asked by: Anna99
We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-11

Can this mouse antibody be used for detection of mouse samples?

Asked by: Anna99
No, this antibody is recommended for use with human samples only.
Answered by: Technical Support 6
Date published: 2017-01-11
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_21759, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 109ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from excellent perfomanceWe received Sc-21759 as a free sample, using 1:500 in milk 5%, O.N. at 4 degrees, with excellent results.
Date published: 2024-06-24
Rated 1 out of 5 by from Did not work at FACSI tried the Ab by FACS at 1 ug/1 milion cells and it did not stain human cells that I know express TLR2 on their surface.
Date published: 2020-10-08
Rated 1 out of 5 by from Indirect FCM on HFF cellsThis antibody was tested by indirect FCM on HFF cells and did not give any signal even at dilution 1:50.
Date published: 2018-11-02
Rated 4 out of 5 by from This antibody is okThis antibody works well even at 1000 dilution and no background.
Date published: 2017-12-05
Rated 5 out of 5 by from This antibody works wellGood antibodies worked in WB and analyzed bands with specific bands and strong bands.
Date published: 2017-10-25
Rated 4 out of 5 by from Strong antibodyIts signals is strong at even 1:1000 dilution and no background.
Date published: 2017-10-04
Rated 1 out of 5 by from Not good as it showedI used this antibody at dilution 1:500 in HT29 cell. It was very hard to tell the exact band in PVDF, the background was very deep, no clear strap was seen even though I tried several times on different cell lysis. Thus, I have to say this antibody is not good as it showed.
Date published: 2017-08-10
Rated 3 out of 5 by from Use it routinely in WBUse it routinely in WB and get two bands，low background
Date published: 2017-07-13
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_21
  • loc_zh_CN, sid_21759, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
TLR2 抗体 (TL2.1) is rated 3.6 out of 5 by 21.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_21
  • loc_zh_CN, sid_21759, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。