TEX14 Antibody (4E4): sc-517070

TEX14 Antibody (4E4) is a mouse monoclonal IgG2a kappa light chain antibody that detects TEX14 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-TEX14 antibody (4E4) is available as the non-conjugated form. TEX14, also known as testis expressed 14 or SGK307 (sugen kinase 307), is a crucial 1,497 amino acid protein belonging to the protein kinase superfamily, primarily expressed in the testis. The gene encoding TEX14 is located on chromosome 17 and plays a vital role in spermatogenesis, particularly in maintaining the normal structure of the intercellular bridge that connects spermatocytes and spermatogonia. This intercellular bridge is essential for the proper development and differentiation of germ cells, ensuring successful sperm production. TEX14 co-localizes with the centralspindlin complex, including MKLP-1 (mitotic kinesin-like protein 1) and male germ cell Rac GTPase, facilitating the conversion of midbody matrix proteins into stable intercellular bridge components. TEX14 features three ankyrin repeats and one protein kinase domain, and exists in three isoforms due to alternative splicing events, which may contribute to functional diversity in spermatogenesis.

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TEX14 Antibody (4E4) References:

1. An abundance of X-linked genes expressed in spermatogonia.  |  Wang, PJ., et al. 2001. Nat Genet. 27: 422-6. PMID: 11279525
2. Sequence and expression of testis-expressed gene 14 (Tex14): a gene encoding a protein kinase preferentially expressed during spermatogenesis.  |  Wu, MH., et al. 2003. Gene Expr Patterns. 3: 231-6. PMID: 12711554
3. TEX14 is essential for intercellular bridges and fertility in male mice.  |  Greenbaum, MP., et al. 2006. Proc Natl Acad Sci U S A. 103: 4982-7. PMID: 16549803
4. Expression profile of male germ cell-associated genes in mouse embryonic stem cell cultures treated with all-trans retinoic acid and testosterone.  |  Silva, C., et al. 2009. Mol Reprod Dev. 76: 11-21. PMID: 18425777
5. Mouse TEX14 is required for embryonic germ cell intercellular bridges but not female fertility.  |  Greenbaum, MP., et al. 2009. Biol Reprod. 80: 449-57. PMID: 19020301
6. TEX14 interacts with CEP55 to block cell abscission.  |  Iwamori, T., et al. 2010. Mol Cell Biol. 30: 2280-92. PMID: 20176808
7. Tex14, a Plk1-regulated protein, is required for kinetochore-microtubule attachment and regulation of the spindle assembly checkpoint.  |  Mondal, G., et al. 2012. Mol Cell. 45: 680-95. PMID: 22405274
8. Novel loci affecting iron homeostasis and their effects in individuals at risk for hemochromatosis.  |  Benyamin, B., et al. 2014. Nat Commun. 5: 4926. PMID: 25352340
9. Dazl determines primordial follicle formation through the translational regulation of Tex14.  |  Rosario, R., et al. 2019. FASEB J. 33: 14221-14233. PMID: 31659914
10. Spermatogonial asynchrony in Tex14 mutant mice lacking intercellular bridges.  |  Rezende-Melo, CA., et al. 2020. Reproduction. 160: 205-215. PMID: 32438343
11. Inhibition mechanism of testis-expressed gene 14 (TEX14) in cytokinetic abscission: Well-tempered metadynamics simulation studies.  |  Cho, Y., et al. 2023. J Chem Phys. 159: PMID: 37409705
12. Correlation between TEX14 and ADAM17 expressions in colorectal cancer tissues of elderly patients and neoplasm staging, invasion, and metastasis.  |  Chen, G., et al. 2024. World J Clin Cases. 12: 5492-5501. PMID: 39188605

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TEX14 Antibody (4E4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TEX14 Antibody (4E4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TEX14 Antibody (4E4) Specifications

Alternate Product Name: TEX14

Product Clone Name: 4E4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1392-1491

Epitope Target: TEX14

Epitope Species: human

Product Reactivity/Detection: TEX14

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TEX14 Antibody (4E4): N/A