Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SQSTM1/p62 Double Nickase Plasmid (m): sc-422075-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SQSTM1/p62 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SQSTM1/p62 Double Nickase Plasmid (m) and SQSTM1/p62 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sqstm1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SQSTM1/p62 Antibody (A-6): sc-48402
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SQSTM1/p62 Double Nickase Plasmid (m) | sc-422075-NIC | 20 µg | $410.00 | |||
SQSTM1/p62 Double Nickase Plasmid (m2) | sc-422075-NIC-2 | 20 µg | $410.00 |
Mouse Sqstm1 encodes SQSTM1/p62, a multifunctional adaptor that binds polyubiquitinated cargo via its UBA domain and links it to LC3-positive autophagosomes through an LIR motif, coordinating selective autophagy and proteostasis. SQSTM1/p62 also scaffolds signaling complexes in the KEAP1–NRF2 oxidative stress response and NF-κB signaling, integrating nutrient sensing and inflammatory cues. As a stress-inducible hub, altered SQSTM1/p62 regulation impacts autophagy flux, aggregate clearance, and mitochondrial quality control, processes frequently interrogated in models of neurodegeneration, metabolic dysfunction, and tumor biology. Its roles in ubiquitin signaling and phase-separated protein assemblies make it a central readout in studies of proteotoxic stress and innate immune signaling.
SQSTM1/p62 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sqstm1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sqstm1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sqstm1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sqstm1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.