Sodium phosphate dibasic dodecahydrate (CAS 10039-32-4)

Sodium phosphate dibasic dodecahydrate is a chemical primarily utilized in scientific research for its buffering properties, essential for maintaining stable pH conditions in various experimental protocols. This compound is particularly important in biochemistry and cell biology where precise pH control is crucial for maintaining the structural integrity and functionality of biological molecules. In biochemical assays, sodium phosphate dibasic dodecahydrate is used to create phosphate buffer solutions, which are vital for enzyme kinetics studies and other enzymatic reactions where pH stability can significantly influence enzymatic activity. Additionally, this buffer system is employed in the preparation of reagents for DNA and RNA extractions, ensuring that these nucleic acids are protected from degradation during processing. The chemical is also frequently used in the formulation of media for cell culture, where it helps regulate the extracellular environment, crucial for cell survival and proliferation. Furthermore, in analytical chemistry, it is used in the calibration of pH meters and as a standard in titration experiments. Through its diverse applications, sodium phosphate dibasic dodecahydrate plays a critical role in advancing fundamental research across multiple scientific disciplines.