SETMAR CRISPR/Cas9 KO Plasmid (h): sc-417901
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SETMAR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SETMAR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SETMAR Antibody (A-12): sc-515243
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SETMAR CRISPR/Cas9 KO Plasmid (h) | sc-417901 | 20 µg | $397.00 |
Overview
SETMAR (also known as Metnase) is a human SET domain–containing lysine methyltransferase fused to a mariner transposase-derived domain that coordinates chromatin modification with DNA end processing. It participates in DNA double-strand break repair and genome maintenance by modulating non-homologous end joining and influencing replication-associated stress responses. Through interactions with repair factors and chromatin components, SETMAR can affect transcriptional regulation and chromatin accessibility at damage sites. Dysregulated SETMAR activity or expression has been associated with altered DNA repair capacity and genomic instability, making it relevant to studies of cancer biology, radiosensitivity, and mechanisms of drug response.
SETMAR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SETMAR gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SETMAR together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SETMAR open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SETMAR protein expression.
This CRISPR knockout system enables efficient generation of SETMAR-deficient cell models for investigation of SETMAR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SETMAR exon(s) critical for SETMAR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SETMAR genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SETMAR CRISPR/Cas9 KO Plasmid (h) and SETMAR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SETMAR locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SETMAR HDR Plasmid (h) and SETMAR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SETMAR homology arms to support homology-directed repair at defined SETMAR target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.