rnk Antibody (464B): sc-101611
- rnk Antibody (464B) is a mouse monoclonal IgG1 rnk antibody, cited in 1 publications, provided at 100 µl ascites
- raised against rnk of E. coli origin
- recommended for detection of rnk of E. coli origin by WB and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for rnk Antibody (464B). This work is in progress.
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rnk Antibody (464B) is a mouse monoclonal IgG1 antibody that detects rnk protein of E. coli origin by western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). Anti-rnk antibody (464B) is a non-conjugated monoclonal isotype antibody that plays a crucial role in understanding transcription regulation in prokaryotic organisms. rnk protein, known as the regulator of nucleoside kinase, consists of 136 amino acids and is structurally similar to Gre factors, which are secondary channel effectors involved in RNA polymerase function. This similarity is significant as rnk competes with E. coli Gre factors and DksA, inhibiting their activity in vitro, thereby influencing transcription regulation. Cellular concentrations of rnk are comparable to those of GreB, suggesting an important role as a regulator of these secondary channel effectors. In vitro studies have shown that rnk does not directly inhibit RNA polymerase′s interaction with promoters or initiate transcript cleavage, highlighting a specific regulatory function rather than a direct inhibitory role. rnk (464B) antibody detection capabilities are essential for researchers studying transcription mechanisms and regulatory protein balance in bacterial systems.
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rnk Antibody (464B) References:
- RNA polymerase structure-function: insights into points of transcriptional regulation. | Severinov, K. 2000. Curr Opin Microbiol. 3: 118-25. PMID: 10744988
- Transcript cleavage factors GreA and GreB act as transient catalytic components of RNA polymerase. | Laptenko, O., et al. 2003. EMBO J. 22: 6322-34. PMID: 14633991
- Effects of DksA, GreA, and GreB on transcription initiation: insights into the mechanisms of factors that bind in the secondary channel of RNA polymerase. | Rutherford, ST., et al. 2007. J Mol Biol. 366: 1243-57. PMID: 17207814
- The carboxy-terminal coiled-coil of the RNA polymerase beta'-subunit is the main binding site for Gre factors. | Vassylyeva, MN., et al. 2007. EMBO Rep. 8: 1038-43. PMID: 17917675
- Crystal structure of Escherichia coli Rnk, a new RNA polymerase-interacting protein. | Lamour, V., et al. 2008. J Mol Biol. 383: 367-79. PMID: 18760284
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
rnk Antibody (464B) | sc-101611 | 100 µl ascites | $339.00 |