RNF10 CRISPR/Cas9 KO Plasmid (h): sc-409761
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF10 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RNF10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF10 CRISPR/Cas9 KO Plasmid (h) | sc-409761 | 20 µg | $397.00 |
Overview
RNF10 encodes a RING-type zinc finger protein implicated in ubiquitin-dependent regulation of protein stability and signaling output. Through E3 ligase-associated processes, RNF10 has been linked to control of transcriptional programs, receptor-mediated signaling, and proteostasis pathways that influence cell growth and differentiation. Altered RNF10 expression or regulation has been reported in contexts involving neural and cardiovascular biology and is studied for its potential contribution to dysregulated cellular homeostasis in disease. RNF10 is therefore relevant for investigating how ubiquitin-mediated remodeling of signaling networks affects gene expression and cell fate decisions.
RNF10 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RNF10 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RNF10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RNF10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RNF10 protein expression.
This CRISPR knockout system enables efficient generation of RNF10-deficient cell models for investigation of RNF10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RNF10 exon(s) critical for RNF10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RNF10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RNF10 CRISPR/Cas9 KO Plasmid (h) and RNF10 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RNF10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RNF10 HDR Plasmid (h) and RNF10 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RNF10 homology arms to support homology-directed repair at defined RNF10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.