RIPA Lysis Buffer System A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included.

RIPA Lysis Buffer System

RIPA Lysis Buffer System is rated 4.8 out of 5 by 34.
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Alternate Names: RIPA Lysis Buffer System also known as RIPA (Radioimmunoprecipitation) Lysis Buffer System
Application: RIPA Lysis Buffer System® is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).

RIPA (Radioimmunoprecipitation) Lysis Buffer System is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. RIPA Lysis Buffer System includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


References

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805

2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318

3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014

4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

Usage :
1. Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.

2. Use 3 ml complete RIPA per gram of tissue

- OR 1 ml complete RIPA per 2.0x107 cells in suspension

- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

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RIPA Lysis Buffer System  Product Citations

See how others have used RIPA Lysis Buffer System. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 2803 total

PMID: # 31279091  Huang, J.|Liu, W.|Doycheva, DM.|Gamdzyk, M.|Lu, W.|Tang, J.|Zhang, JH.| et al. 2019. Free Radic. Biol. Med. 141: 322-337.

PMID: # 30141211  Maretti-Mira, AC.|Wang, X.|Wang, L.|DeLeve, LD.| et al. 2019. Hepatology. 69: 1259-1272.

PMID: # 31575039  2019. Int J Mol Sci. 20:

PMID: # 31545452  2019. Oncol. Rep.

PMID: # 30835049  Kalantary-Charvadeh, A.|Sanajou, D.|Hemmati-Dinarvand, M.|Marandi, Y.|Khojastehfard, M.|Hajipour, H.|Mesgari-Abbasi, M.|Roshangar, L.|Nazari Soltan Ahmad, S.| et al. 2019. Cardiovasc. Toxicol.

PMID: # 31325432  Agrawal, L.|Vimal, SK.|Shiga, T.| et al. 2019. Neuropharmacology. 158: 107712.

PMID: # 31278615  2019. Cardiovasc. Toxicol.

PMID: # 31241772  Mansoori, B.|Mohammadi, A.|Naghizadeh, S.|Gjerstorff, M.|Shanehbandi, D.|Shirjang, S.|Najafi, S.|Holmskov, U.|Khaze, V.|Duijf, PHG.|Baradaran, B.| et al. 2019. J. Cell. Physiol.

PMID: # 31114375  Rong, X.|Han, Q.|Lin, X.|Kremerskothen, J.|Wang, E.| et al. 2019. Cancer Manag Res. 11: 3395-3410.

PMID: # 31273064  Jain, A.|Agostini, LC.|McCarthy, GA.|Chand, SN.|Ramirez, A.|Nevler, A.|Cozzitorto, J.|Schultz, CW.|Lowder, CY.|Smith, KM.|Waddell, ID.|Raitses-Gurevich, M.|Stossel, C.|Gorman, YG.|Atias, D.|Yeo, CJ.|Winter, JM.|Olive, KP.|Golan, T.|Pishvaian, MJ.|Ogilvie, D.|James, DI.|Jordan, AM.|Brody, JR.| et al. 2019. Cancer Res.

Citations 1 to 10 of 2803 total

What detergents does this product contain?

Asked by: ksk421
Thank you for your question. The RIPA Lysis buffer (sc-24948) includes two ionic detergents and one nonionic detergent in Tris buffer. Here is the composition of the lysis buffer component (1x Lysis Buffer): 1x TBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 0.004% sodium azide.
Answered by: Technical Support
Date published: 2020-07-23

Does RIPA buffer system contain EDTA in the lysis buffer?

Asked by: Yanning
Thank you for your question. No, the lysis buffer in the RIPA buffer does not contain EDTA.
Answered by: Tech Service
Date published: 2020-02-07

What are the components in the buffer and at what concentration, pH value? thanks!

Asked by: Davidxu
Thank you for your question. The general components can be found above in the product description and on the datasheet that accompanies the product. Please reach out to Technical service if additional information is needed. Here is a link to a sample lot CoA: http://datasheets.scbt.com/coa/coa.php?sc=sc-24948A&lot=H2319
Answered by: Tech Service
Date published: 2020-02-06

PODRÍA DECIRME EL % DE SDS QUE LLEVA? GRACIAS.

Asked by: KELAMAY
Thank you for your question. This RIPA lysis buffer system contains 0.1% SDS.
Answered by: Tech Service
Date published: 2020-02-04

Is it normal that I get precipitation when I prepare the complete RIPA buffer?

Asked by: Ain Ayob
Thank you for your question. There shouldn't be any precipitation. If there is any precipitation, it should dissolve if heating up the solution to room temperature. If this still does not resolve the issue, please reach out to Technical Service.
Answered by: Tech Service
Date published: 2020-01-22

Buenos días, necesitaría saber si entre el cóctel de inhibidores que acompaña el kit de RIPA se encuentra E-64 o al menos algún inhibidor de cisteína-proteasas equivalente. Gracias.

Asked by: ebarrofe
Thank you for your question. We can confirm that there is a cysteine protease inhibitor in the cocktail, but further information regarding the protease inhibitors is proprietary.
Answered by: Tech Service
Date published: 2020-01-22

Buenos días, Hemos adquirido el RIPA buffer con código: sc-24948. Necesitaría saber la composición específica del buffer. Muchas gracias. Saludos. Raquel

Asked by: Raquel83
Thank you for your question. The composition of the vials are up on the top of the page: https://www.scbt.com/p/ripa-lysis-buffer-system?requestFrom=search VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1) VIAL 2: 500 μL (200mM) PMSF in DMSO VIAL 3: 500 μL protease inhibitor cocktail in DMSO VIAL 4: 500 μL (100mM) sodium orthovanadate in water sc-24948A, 500 mL - Components supplied in four vials: VIAL 1: 500 mL 1X lysis buffer VIAL 2: 5 ml (200mM) PMSF in DMSO VIAL 3: 5 ml protease inhibitor cocktail in DMSO VIAL 4: 5 ml (100mM) sodium orthovanadate in water
Answered by: Tech Service
Date published: 2020-01-08

How long is the quanlity guarantee period of this RIPA Lysis Buffer?

Asked by: Houmin
Thank you for your question. All Santa Cruz biotechnology products are warrantied for 1 year from the date of purchase.
Answered by: Tech Service
Date published: 2019-09-13
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Rated 5 out of 5 by from Good quality and good price RIPA buffer containing protease and phosphatase inhibitors. It works well for me.
Date published: 2020-05-16
Rated 5 out of 5 by from 很好 半 年 前 买 了 500 ml, 用 起 来 感 觉 很 不 错,准 备 再 买 一 瓶!极 力 推 荐 啊!
Date published: 2020-05-12
Rated 5 out of 5 by from Working well this inhibitor works well the cost is low the ripa lysis buffer set is convenient
Date published: 2020-02-26
Rated 4 out of 5 by from Indispensable buffer Cheaper than that from other vendors. It worked good in our hands when we used it to measure TNF-α concentration in liver lysate.
Date published: 2020-01-23
Rated 5 out of 5 by from good product works well for my western blot experiments. will recommend this
Date published: 2019-05-26
Rated 5 out of 5 by from Works great! Works great for lysing mammalian cell for western blots. No slimy/viscous pellets in the lysate.
Date published: 2018-10-30
Rated 5 out of 5 by from Great cost performance Although this RIPA lysis buffer kit contains protease inhibitor cocktail and phosphatase inhibitors, cost is inexpensive! Of course, it works well!
Date published: 2018-02-22
Rated 4 out of 5 by from how to make it working 1x ripa buffer as per its mentioned that for 1 ml we have to add particular content..do we have to reduce the amount of other content from the 1 ml and then add or in 1 ml we can add 10 ul of each component???
Date published: 2017-12-17
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