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Ribosomal Protein S29 Antibody (3G9): sc-517071

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Datasheets
  • Ribosomal Protein S29 Antibody (3G9) is a mouse monoclonal IgG2a κ Ribosomal Protein S29 antibody provided at 100 µg/ml
  • raised against amino acids 1-56 representing partial length Ribosomal Protein S29 of human origin
  • recommended for detection of Ribosomal Protein S29 of mouse, rat and human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Ribosomal Protein S29 Antibody (3G9). This work is in progress.

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    Ribosomal Protein S29 Antibody (3G9) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ribosomal Protein S29 (RPS29) of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RPS29 antibody (3G9) is available as the non-conjugated format. RPS29 is a crucial component of the 40S ribosomal subunit, consisting of 56 amino acids and featuring a C2-C2 zinc finger-like domain. RPS29 is primarily located in the cytoplasm, where RPS29 plays a vital role in protein synthesis by facilitating ribosome assembly, essential for translating mRNA into proteins. Proper ribosome functioning is critical for cellular health, as ribosomes are responsible for synthesizing proteins that perform various functions within cells. RPS29 binds zinc as a cofactor, which is important for structural integrity and function. RPS29 enhances tumor suppressor activity of Rap 1A, suggesting a potential role in tumor suppression. Like many ribosomal proteins, RPS29 is encoded by multiple processed pseudogenes scattered throughout the genome, reflecting the complexity and evolutionary significance of ribosomal protein genes.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ribosomal Protein S29 Antibody (3G9) References:

    1. Regulation of the quiescence-induced genes: quiescin Q6, decorin, and ribosomal protein S29.  |  Coppock, D., et al. 2000. Biochem Biophys Res Commun. 269: 604-10. PMID: 10708601
    2. Differential expression of genes coding for ribosomal proteins in different human tissues.  |  Bortoluzzi, S., et al. 2001. Bioinformatics. 17: 1152-7. PMID: 11751223
    3. Low content of protein S29 in ribosomes of human lung cancer cell line a549: detected by two-dimensional electrophoresis.  |  Zhou, ZD., et al. 2003. Protein Pept Lett. 10: 91-7. PMID: 12625830
    4. S29 ribosomal protein induces apoptosis in H520 cells and sensitizes them to chemotherapy.  |  Khanna, N., et al. 2003. Biochem Biophys Res Commun. 304: 26-35. PMID: 12705879
    5. Cloning, sequencing and expression of the L5, L21, L27a, L28, S5, S9, S10 and S29 human ribosomal protein mRNAs.  |  Frigerio, JM., et al. 1995. Biochim Biophys Acta. 1262: 64-8. PMID: 7772601
    6. Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry.  |  Vladimirov, SN., et al. 1996. Eur J Biochem. 239: 144-9. PMID: 8706699
    7. Structure and evolution of mammalian ribosomal proteins.  |  Wool, IG., et al. 1995. Biochem Cell Biol. 73: 933-47. PMID: 8722009
    8. The S29 ribosomal protein increases tumor suppressor activity of K rev-1 gene on v-K ras-transformed NIH3T3 cells.  |  Kondoh, N., et al. 1996. Biochim Biophys Acta. 1313: 41-6. PMID: 8781548
    9. A map of 75 human ribosomal protein genes.  |  Kenmochi, N., et al. 1998. Genome Res. 8: 509-23. PMID: 9582194

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Ribosomal Protein S29 Antibody (3G9)

    sc-517071
    100 µg/ml
    $316.00

    I am using Ribosomal Protein S29 (3G9): sc-517071 for immunofluorescence, what dilution of this antibody should I use?

    Asked by: jerojero
    Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 5 out of 5 by from Good for Western blotAntibody detects human recombinant Ribosomal Protein S29 by Western blot. -SCBT QC
    Date published: 2023-09-14
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    Ribosomal Protein S29 Antibody (3G9) is rated 5.0 out of 5 by 1.
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