Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RBM24 CRISPR/Cas9 KO Plasmid (h): sc-410387
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RBM24 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RBM24 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RBM24 CRISPR/Cas9 KO Plasmid (h) | sc-410387 | 20 µg | $397.00 |
Overview
RBM24 (RNA binding motif protein 24) is a muscle-enriched RNA-binding protein that regulates post-transcriptional gene expression by controlling alternative splicing, mRNA stability, and transcript localization. It contributes to myogenic differentiation and sarcomere organization through coordination of muscle-specific RNA programs and cross-talk with splicing and translation machinery. RBM24 activity has been linked to cardiac and skeletal muscle development, and altered RBM24-dependent RNA processing is associated with cardiomyopathy-related phenotypes and dysregulated differentiation states. Because RBM24 modulates isoform choice and RNA fate, it is frequently studied in pathways governing cytoskeletal assembly, excitation–contraction coupling, and stress-responsive remodeling.
RBM24 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RBM24 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RBM24 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RBM24 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RBM24 protein expression.
This CRISPR knockout system enables efficient generation of RBM24-deficient cell models for investigation of RBM24 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RBM24 exon(s) critical for RBM24 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RBM24 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RBM24 CRISPR/Cas9 KO Plasmid (h) and RBM24 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RBM24 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RBM24 HDR Plasmid (h) and RBM24 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RBM24 homology arms to support homology-directed repair at defined RBM24 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.