Date published: 2026-3-17

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Rapamycin (CAS 53123-88-9)

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Alternate Names:
Rapamycin is also known as mTOR Inhibitor I.
Application:
Rapamycin acts as an allosteric inhibitor of mTOR and inhibits FRAP autophosphorylation, which leads to activation of the p70/85 S6 kinase.
CAS Number:
53123-88-9
Purity:
≥98%
Molecular Weight:
914.17
Molecular Formula:
C51H79NO13
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Rapamycin is a FRAP inhibitor that exhibits binding and inhibitory actions to the FK506 binding protein (FKBP5) proline rotamase via simultaneous binding by FKBP12 and FRAP. FRAP (RAFT1) proteins exhibit homology to PI 4- and PI 3-kinases, which have PI 4-kinase and autophosphorylating activities. The rapamycin/FKBP complex does not inhibit the FRAP PI 4-kinase activity, but does inhibit FRAP autophosphorylation. Rapamycin is unique in its ability to inhibit lymphokine induced cell proliferation at the G1 and S phase as well as an irreversible cellular arrest at the G1 phase in S. cerevisiae cells. Rapamycin also exhibits selective signal blocking leading to the activation of p70/85 S6 kinase, which is potentially due to the inhibition of FRAP autophosphorylation or protein kinase activity. Rapamycin also exhibits Angiogenesis inhibition, possibly through the inhibition of the Akt pathway. Rapamycin is an inhibitor of mTOR. Rapamycin is also known as Rapamune, 23,27-Epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine, AY 22989, and mTOR Inhibitor I.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Rapamycin (CAS 53123-88-9) References

  1. Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases.  |  Chung, J., et al. 1992. Cell. 69: 1227-36. PMID: 1377606
  2. Rapamycin-induced inhibition of the 70-kilodalton S6 protein kinase.  |  Price, DJ., et al. 1992. Science. 257: 973-7. PMID: 1380182
  3. Pathological angiogenesis is induced by sustained Akt signaling and inhibited by rapamycin.  |  Phung, TL., et al. 2006. Cancer Cell. 10: 159-70. PMID: 16904613
  4. Inhibition of T and B lymphocyte proliferation by rapamycin.  |  Kay, JE., et al. 1991. Immunology. 72: 544-9. PMID: 1709916
  5. FK506 and rapamycin: novel pharmacological probes of the immune response.  |  Chang, JY., et al. 1991. Trends Pharmacol Sci. 12: 218-23. PMID: 1710854
  6. Targets for cell cycle arrest by the immunosuppressant rapamycin in yeast.  |  Heitman, J., et al. 1991. Science. 253: 905-9. PMID: 1715094
  7. Rapamycin: An InhibiTOR of Aging Emerges From the Soil of Easter Island.  |  Arriola Apelo, SI. and Lamming, DW. 2016. J Gerontol A Biol Sci Med Sci. 71: 841-9. PMID: 27208895
  8. Rapamycin rejuvenates oral health in aging mice.  |  An, JY., et al. 2020. Elife. 9: PMID: 32342860
  9. RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs.  |  Sabatini, DM., et al. 1994. Cell. 78: 35-43. PMID: 7518356
  10. Control of p70 s6 kinase by kinase activity of FRAP in vivo.  |  Brown, EJ., et al. 1995. Nature. 377: 441-6. PMID: 7566123
  11. The rapamycin and FKBP12 target (RAFT) displays phosphatidylinositol 4-kinase activity.  |  Sabatini, DM., et al. 1995. J Biol Chem. 270: 20875-8. PMID: 7673106
  12. Dissociation of pp70 ribosomal protein S6 kinase from insulin-stimulated glucose transport in 3T3-L1 adipocytes.  |  Fingar, DC., et al. 1993. J Biol Chem. 268: 3005-8. PMID: 7679106
  13. A mammalian protein targeted by G1-arresting rapamycin-receptor complex.  |  Brown, EJ., et al. 1994. Nature. 369: 756-8. PMID: 8008069
  14. Rapamycin-induced inhibition of p34cdc2 kinase activation is associated with G1/S-phase growth arrest in T lymphocytes.  |  Morice, WG., et al. 1993. J Biol Chem. 268: 3734-8. PMID: 8429048
  15. [Pulmonary thromboembolism in autopsy material of the Department of Pathological Anatomy of the Warsaw Medical Academy].  |  Gawdzińska-Grabowska, H. 1977. Pol Tyg Lek. 32: 679-82. PMID: 866250

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Rapamycin, 1 mg

sc-3504
1 mg
$63.00

Rapamycin, 5 mg

sc-3504A
5 mg
$158.00

Rapamycin, 25 mg

sc-3504B
25 mg
$326.00

How many in bottle?

Asked by: Seeker
Thank you for your question . The current sizes we offer are 1mg, 5mg and 25mg .
Answered by: BlakeJ
Date published: 2024-03-28

I’m in the process of ordering the 5mg dosage of rapamycin from your site, is it a 30 day supply? How many tablets come in the order? There is nothing listed that gives an amount of tablets in the order. Thanks 

Asked by: Napthyl
Thank you for your question. Santa Cruz Biotechnology products are for research use only. Our products are not for diagnostic or therapeutic use.
Answered by: BlakeJ
Date published: 2024-03-28

I'm interested in purchasing rapamycin 5mg, which according to your catalogue will be available in October.  How many 5mg tabs are there per bottle?  Should I pre-order or wait till they're in stock?  Thanks

Asked by: Fenton
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2024-03-28

What concentration will induce autophagy in cell (CHO, HeLa, HepG2) culture?

Asked by: Jessielulu
Thank you for your question. We have not performed any cell assays with these cell types, so we cannot provide information on the concentration necessary for inducing autophagy.
Answered by: Tech Support Europe
Date published: 2023-03-03

Hi can you please explain in what form this is delivered? İf I order say 25mg rapamycin, is it easily divisible into smaller doses?

Asked by: Research123
Thank you for your question. This is provided as a solid. It is soluble in DMSO (25 mg/ml), ethanol (up to 50 mg/ml), chloroform (5 mg/ml), methanol (25 mg/ml), ether, and acetone. Insoluble in water. We recommend preparing a solution just prior to use. Upon resuspension, Rapamycin should be aliquoted and stored at -20°C. Avoid repeated freeze-thaw cycles. Resuspended product should be stable for 3 months at -20°C when properly stored. Please keep in mind some additives may effect stability.
Answered by: Technical Support
Date published: 2023-11-07

Where are you shipping from?

Asked by: kevinp
Thank you for your question. For accurate availability, shipping and pricing information regarding our products, please contact Customer Service.
Answered by: Tech Service
Date published: 2023-01-26

溶解药品时要把药品拿出来溶解还是直接在棕色瓶子里溶解,如果在棕色瓶子里溶解的话之后取样瓶子应该会有很多残留吧。药品需要避光保存么?

Asked by: 小静静123
感谢您的提问。这支瓶子是一个4ml的琥珀色玻璃瓶,sc-3504是建议避光保存的。溶解的时候您可以先加入一定体积的溶解液,充分溶解后倒出溶液,再加入溶解液洗瓶,收集洗涤sc-3504瓶子的溶液,并补充至最终的稀释液体积,获得您需要的浓度。更多问题,欢迎通过:电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. We do recommend protecting from light. It is packaged in 4 ml amber glass vial. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2022-11-30

一毫克用多少DMSO 溶解不伤害细胞呢?

Asked by: 小静静123
不同的细胞,DMSO溶解指数可能不一样, 具体需要参照相关文献, 或者通过实验摸索到最佳的用量。
Answered by: Technical Support 2
Date published: 2022-11-30
  • y_2026, m_3, d_16, h_6CST
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Rated 5 out of 5 by from Rapamycin indirectly impacts O-GlcNAcylationTreatments of normal and colon cancer cells with mTOR regulators including rapamycin modulate O-GlcNAc transferase and O-GlcNAcylation levels. However, deregulation of O-GlcNAcylation affects mTOR signaling activation only in cancer cells. Thus, a crosstalk occurs between O-GlcNAcylation and mTOR signaling.
Date published: 2019-03-26
Rated 5 out of 5 by from Provided all essential information alongProvided all essential information along with nominal price
Date published: 2016-01-28
Rated 5 out of 5 by from Cells were preCells were pre-treated with rapamycin (sc-3504) for 30 minutes prior to TPO stimulation, then screened for proliferation by BrdU staining and FCM (16123382). -SCBT Publication Review
Date published: 2015-07-02
Rated 5 out of 5 by from KanayamaKanayama, et. al. (PubMed ID 25609235) used rapamycin (sc-3504) to induce autophagy in the macrophages studied. -SCBT Publication Review
Date published: 2015-07-02
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Rapamycin is rated 5.0 out of 5 by 4.
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