Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RAP1 CRISPR/Cas9 KO Plasmid (h): sc-401709
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RAP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RAP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RAP1 Antibody (4C8/1): sc-53434
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RAP1 CRISPR/Cas9 KO Plasmid (h) | sc-401709 | 20 µg | $397.00 |
Overview
TERF2IP encodes RAP1, a core component of the shelterin complex that associates with telomeric repeat–binding factor 2 (TRF2) to help protect chromosome ends from inappropriate DNA damage signaling and end-to-end fusions. Beyond telomere end protection, RAP1 contributes to telomere length homeostasis, replication stress responses at telomeres, and broader genome stability through coordination of DNA repair and checkpoint pathways. RAP1 has also been implicated in transcriptional regulation at extra-telomeric sites, linking telomere biology to chromatin and gene expression programs. Dysregulation of TERF2IP/RAP1 function is relevant to telomere dysfunction phenotypes, genomic instability, and cellular transformation processes studied in cancer and aging-related models.
RAP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TERF2IP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TERF2IP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TERF2IP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RAP1 protein expression.
This CRISPR knockout system enables efficient generation of TERF2IP-deficient cell models for investigation of RAP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TERF2IP exon(s) critical for RAP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TERF2IP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RAP1 CRISPR/Cas9 KO Plasmid (h) and RAP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TERF2IP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RAP1 HDR Plasmid (h) and RAP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TERF2IP homology arms to support homology-directed repair at defined TERF2IP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.