QPCT Antibody (4E11): sc-517122
- QPCT Antibody (4E11) is a mouse monoclonal IgG2a κ QPCT antibody, cited in 1 publications, provided at 100 µg/ml
- raised against amino acids 262-359 representing partial length QPCT of human origin
- recommended for detection of QPCT of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for QPCT Antibody (4E11). This work is in progress.
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QPCT Antibody (4E11) is a mouse monoclonal IgG2a kappa light chain antibody that detects the QPCT protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-QPCT antibody (4E11) is available as the non-conjugated format. QPCT, or glutaminyl-peptide cyclotransferase, is a crucial 361 amino acid enzyme that plays a significant role in the post-translational modification of neuroendocrine peptides by facilitating the formation of pyroglutamyl residues. This modification is essential for the stability and biological activity of these peptides, which are involved in various physiological processes, including hormone regulation and neurotransmission. QPCT binds one zinc ion per subunit, which is vital for enzymatic activity, and exhibits a preference against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue. Human QPCT shares a remarkable 86% overall sequence identity with its bovine counterpart, highlighting evolutionary conservation. QPCT contains an N-terminal signal peptide region, multiple glycosylation and phosphorylation sites, and two cysteine residues that are conserved between the bovine and human enzymes. QPCT exists in two alternatively spliced isoforms and is conserved across various species, including chimpanzee, dog, cow, mouse, rat, chicken, fruit fly, mosquito, Magnaporthe grisea, and Neurospora crassa, mapping to human chromosome 2p22.2.
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QPCT Antibody (4E11) References:
- Glutaminyl cyclases unfold glutamyl cyclase activity under mild acid conditions. | Schilling, S., et al. 2004. FEBS Lett. 563: 191-6. PMID: 15063747
- Association of multiple nucleotide variations in the pituitary glutaminyl cyclase gene (QPCT) with low radial BMD in adult women. | Ezura, Y., et al. 2004. J Bone Miner Res. 19: 1296-301. PMID: 15231017
- Generation and annotation of the DNA sequences of human chromosomes 2 and 4. | Hillier, LW., et al. 2005. Nature. 434: 724-31. PMID: 15815621
- The association of common polymorphisms in the QPCT gene with bone mineral density in the Chinese population. | Huang, QY. and Kung, AWC. 2007. J Hum Genet. 52: 757-762. PMID: 17687619
- Isolation of an isoenzyme of human glutaminyl cyclase: retention in the Golgi complex suggests involvement in the protein maturation machinery. | Cynis, H., et al. 2008. J Mol Biol. 379: 966-80. PMID: 18486145
- Glutaminyl cyclase inhibition attenuates pyroglutamate Abeta and Alzheimer's disease-like pathology. | Schilling, S., et al. 2008. Nat Med. 14: 1106-11. PMID: 18836460
- An enzyme(s) that converts glutaminyl-peptides into pyroglutamyl-peptides. Presence in pituitary, brain, adrenal medulla, and lymphocytes. | Busby, WH., et al. 1987. J Biol Chem. 262: 8532-6. PMID: 3597387
- Molecular cloning, sequence analysis and expression of human pituitary glutaminyl cyclase. | Song, I., et al. 1994. J Mol Endocrinol. 13: 77-86. PMID: 7999256
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
QPCT Antibody (4E11) | sc-517122 | 100 µg/ml | $322.00 |