PRDM14 Antibody (F-10): sc-518186
- PRDM14 Antibody (F-10) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 409-429 of PRDM14 of human origin
- recommended for detection of PRDM14 of human origin by WB, IP, IF and ELISA
- Anti-PRDM14 Antibody (F-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PRDM14 Antibody (F-10) for WB applications. These reagents are now offered in bundles with PRDM14 Antibody (F-10) (see ordering information below).
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PRDM14 Antibody (F-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects PRDM14 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PRDM14 monoclonal antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRDM14, also known as PFM11, is a 571 amino acid protein that plays a crucial role in transcriptional regulation and is primarily located in the nucleus. PRDM14′s structure includes six C2H2-type zinc fingers and one SET domain, which are essential for modulating gene expression. These structural features allow PRDM14 to interact with specific DNA sequences, influencing the transcription of target genes involved in cell differentiation and tumorigenesis. Dysregulation of PRDM14 has been implicated in various cancers, where the balance between PR-plus and PR-minus isoforms can determine malignancy. PR-plus isoform is often underexpressed or disrupted in cancer cells, while PR-minus isoform is typically overexpressed, highlighting PRDM14′s importance in cancer biology and potential as a therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRDM14 Antibody (F-10) References:
- The retinoblastoma protein-interacting zinc finger gene RIZ in 1p36-linked cancers. | Huang, S. 1999. Front Biosci. 4: D528-32. PMID: 10369808
- The yin-yang of PR-domain family genes in tumorigenesis. | Jiang, GL. and Huang, S. 2000. Histol Histopathol. 15: 109-17. PMID: 10668202
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. | Strausberg, RL., et al. 2002. Proc Natl Acad Sci U S A. 99: 16899-903. PMID: 12477932
- Why mice have lost genes for COL21A1, STK17A, GPR145 and AHRI: evidence for gene deletion at evolutionary breakpoints in the rodent lineage. | Fitzgerald, J. and Bateman, JF. 2004. Trends Genet. 20: 408-12. PMID: 15313548
- Essential roles of a zebrafish prdm1/blimp1 homolog in embryo patterning and organogenesis. | Wilm, TP. and Solnica-Krezel, L. 2005. Development. 132: 393-404. PMID: 15623803
- Family expansion and gene rearrangements contributed to the functional specialization of PRDM genes in vertebrates. | Fumasoni, I., et al. 2007. BMC Evol Biol. 7: 187. PMID: 17916234
- Gene amplification and overexpression of PRDM14 in breast cancers. | Nishikawa, N., et al. 2007. Cancer Res. 67: 9649-57. PMID: 17942894
- PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells. | Tsuneyoshi, N., et al. 2008. Biochem Biophys Res Commun. 367: 899-905. PMID: 18194669
- The retinoblastoma interacting zinc finger gene RIZ produces a PR domain-lacking product through an internal promoter. | Liu, L., et al. 1997. J Biol Chem. 272: 2984-91. PMID: 9006946
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRDM14 Antibody (F-10) | sc-518186 | 200 µg/ml | $316.00 | |||
PRDM14 Antibody (F-10): m-IgG Fc BP-HRP Bundle | sc-550886 | 200 µg Ab; 10 µg BP | $354.00 | |||
PRDM14 Antibody (F-10): m-IgG2a BP-HRP Bundle | sc-550885 | 200 µg Ab; 10 µg BP | $354.00 | |||
PRDM14 Antibody (F-10) AC | sc-518186 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PRDM14 Antibody (F-10) HRP | sc-518186 HRP | 200 µg/ml | $316.00 | |||
PRDM14 Antibody (F-10) FITC | sc-518186 FITC | 200 µg/ml | $330.00 | |||
PRDM14 Antibody (F-10) PE | sc-518186 PE | 200 µg/ml | $343.00 | |||
PRDM14 Antibody (F-10) Alexa Fluor® 488 | sc-518186 AF488 | 200 µg/ml | $357.00 | |||
PRDM14 Antibody (F-10) Alexa Fluor® 546 | sc-518186 AF546 | 200 µg/ml | $357.00 | |||
PRDM14 Antibody (F-10) Alexa Fluor® 594 | sc-518186 AF594 | 200 µg/ml | $357.00 | |||
PRDM14 Antibody (F-10) Alexa Fluor® 647 | sc-518186 AF647 | 200 µg/ml | $357.00 | |||
PRDM14 Antibody (F-10) Alexa Fluor® 680 | sc-518186 AF680 | 200 µg/ml | $357.00 | |||
PRDM14 Antibody (F-10) Alexa Fluor® 790 | sc-518186 AF790 | 200 µg/ml | $357.00 |