PP2B-Aα CRISPR/Cas9 KO Plasmid (m): sc-422383
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PP2B-Aα CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PP2B-Aα genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PP2B-Aα Antibody (D-9): sc-17808
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP2B-Aα CRISPR/Cas9 KO Plasmid (m) | sc-422383 | 20 µg | $397.00 |
Overview
Ppp3ca encodes the catalytic Aα subunit of protein phosphatase 2B (PP2B-Aα/calcineurin), a Ca²⁺/calmodulin-dependent serine/threonine phosphatase that couples intracellular calcium signals to transcriptional and cytoskeletal programs. In mouse cells, calcineurin dephosphorylates key substrates such as NFAT transcription factors, shaping calcium-dependent gene expression, immune signaling, and activity-dependent neuronal processes including synaptic plasticity. PP2B-Aα participates in pathways controlling ion channel regulation, mitochondrial and metabolic adaptation, and stress-responsive signaling networks. Dysregulated calcineurin activity has been linked to altered immune cell activation, neurodevelopmental and neurodegenerative phenotypes, and maladaptive cardiac and skeletal muscle remodeling in experimental models.
PP2B-Aα CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ppp3ca gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppp3ca together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppp3ca open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PP2B-Aα protein expression.
This CRISPR knockout system enables efficient generation of Ppp3ca-deficient cell models for investigation of PP2B-Aα signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppp3ca exon(s) critical for PP2B-Aα function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppp3ca genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PP2B-Aα CRISPR/Cas9 KO Plasmid (m) and PP2B-Aα CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppp3ca locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PP2B-Aα HDR Plasmid (m) and PP2B-Aα HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppp3ca homology arms to support homology-directed repair at defined Ppp3ca target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.