PMSF Solution (CAS 329-98-6)

PMSF (phenylmethylsulfonyl fluoride) solution is a potent irreversible serine protease inhibitor widely used in biochemical and molecular biology research. Its mechanism of action involves the covalent modification of the active site serine residue in serine proteases, thereby inhibiting their activity. PMSF is commonly used to inhibit serine proteases such as trypsin, chymotrypsin, and thrombin during protein purification and isolation procedures. By preventing protease activity, PMSF helps maintain the integrity and stability of proteins during extraction and purification processes, ensuring the preservation of protein structure and function. Additionally, PMSF is employed to inhibit endogenous proteases present in biological samples, such as cell lysates and tissue homogenates, thus enabling the study of specific protein-protein interactions, enzyme activities, and signaling pathways. Moreover, PMSF is utilized in the preparation of protein extracts for downstream applications such as enzyme assays, Western blotting, and mass spectrometry analysis. Its ability to selectively inhibit serine proteases makes PMSF a valuable tool in various research applications, including enzymology, protein biochemistry, and proteomics. As a reversible inhibitor, PMSF offers researchers precise control over protease activity, facilitating the investigation of biological processes and molecular mechanisms underlying cellular functions.