PLA2R Double Nickase Plasmid (h): sc-411636-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PLA2R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PLA2R Double Nickase Plasmid (h) and PLA2R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PLA2R1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PLA2R Double Nickase Plasmid (h) | sc-411636-NIC | 20 µg | $410.00 | |||
PLA2R Double Nickase Plasmid (h2) | sc-411636-NIC-2 | 20 µg | $410.00 |
PLA2R1 encodes the M-type phospholipase A2 receptor (PLA2R), a single-pass transmembrane C-type lectin family member that binds secreted phospholipase A2 enzymes and other extracellular ligands to regulate receptor-mediated endocytosis, ligand clearance, and downstream signaling. Through its extracellular recognition domains, PLA2R influences inflammatory lipid mediator pathways and cellular responses linked to membrane remodeling and oxidative stress. PLA2R1 expression and receptor turnover have been associated with immune-related tissue injury and organ-specific inflammation, supporting its use as a molecular entry point for studying receptor–ligand dynamics in disease-relevant contexts. In renal biology, PLA2R is a key antigenic target in autoimmune glomerular disorders, making PLA2R1 perturbation useful for mechanistic studies of antigen presentation, antibody binding, and cell stress responses.
PLA2R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PLA2R1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PLA2R1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PLA2R1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PLA2R1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.