PKC θ Pseudo-substrate inhibitor is a protein kinase Cθ pseudosubstrate peptide that can be used as a competitive inhibitor for in vitro PKC θ kinase assays.
1. Osada, S., Mizuno, K., Saido, T.C., Suzuki, K., Kuroki, T. and Ohno, S. 1992. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle. Mol. Cell. Biol. 12(9): 3930-3938. PMID: 1508194
Recommended reaction conditions: PKCθ-containing sample is incubated at 30 - 37°C, with or without 400 μM PKC θ Pseudo-substrate inhibitor, in 50 mM Tris buffer (pH 7.5) containing 5 mM MgCl2, 0.1 mM Na3VO4, 0.1 mM Na4P2O7, 1 mM NaF, 0.1 mM PMSF, 50 μM ATP, 5 μCi [γ-32P]ATP, 4 μg phosphatidylserine, and 40 μM PKCθ substrate (sc-3107).
Storage:Protect from moisture. After reconstitution, aliquot and freeze (-20°C). This product is stable for 2 years as supplied. Stock solutions are stable for 6 months -20°C.
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Various publications cite the use of PKC PseudoVarious publications cite the use of PKC Pseudo-substrate inhibitor, a protein kinase C pseudosubstrate peptide, as a competitive inhibitor for in vitro PKC kinase assays. -SCBT Publication Review