PIWIL1 Double Nickase Plasmid (h): sc-418611-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PIWIL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PIWIL1 Double Nickase Plasmid (h) and PIWIL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PIWIL1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIWIL1 Double Nickase Plasmid (h) | sc-418611-NIC | 20 µg | $410.00 | |||
PIWIL1 Double Nickase Plasmid (h2) | sc-418611-NIC-2 | 20 µg | $410.00 |
PIWIL1 (HIWI) is a PIWI subfamily Argonaute protein that binds piRNAs to regulate post-transcriptional gene silencing, transposon repression, and epigenetic control of genome stability, particularly in germline-associated programs. Through interactions with piRNA biogenesis machinery and chromatin-associated factors, PIWIL1 contributes to RNA-guided regulation of repetitive elements and mRNA targets, influencing cell fate decisions and differentiation. Dysregulated PIWIL1 expression has been reported in multiple cancers and is studied in the context of tumor cell proliferation, stem-like phenotypes, and altered small-RNA regulatory networks. These functions make PIWIL1 a useful target for investigating piRNA pathway biology, genome integrity maintenance, and RNA-mediated regulatory mechanisms in human cells.
PIWIL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PIWIL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PIWIL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PIWIL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PIWIL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.