Date published: 2025-9-30

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PIPES, Free Acid (CAS 5625-37-6)

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Alternate Names:
1,4-Piperazinediethanesulfonic acid; Piperazine-N,N′-bis(2-ethanesulfonic acid); 2,2′-(Piperazine-1,4-diyl)diethanesulfonic acid
Application:
PIPES, Free Acid is a buffering agent with a pKa near physiological pH
CAS Number:
5625-37-6
Purity:
≥99%
Molecular Weight:
302.4
Molecular Formula:
C8H18N2O6S2
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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PIPES, Free Acid [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES, Free Acid has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Additional forms available: PIPES, Sesquisodium Salt (sc-216100) PIPES dipotassium salt (sc-258019) PIPES disodium salt (sc-264078) PIPES, Sodium Salt (sc-296097). Buffers can be prepared by adding a solution of base to PIPES, Free Acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.


PIPES, Free Acid (CAS 5625-37-6) References

  1. The influence of the buffer on maintenance of tissue lipid in specimens for scanning electron microscopy.  |  Schiff, RI. and Gennaro, JF. 1979. Scan Electron Microsc. 449-58. PMID: 118516
  2. Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.  |  Babich, V., et al. 2009. J Biol Chem. 284: 12459-68. PMID: 19258324
  3. A chimeric kinesin-1 head/kinesin-5 tail motor switches between diffusive and processive motility.  |  Thiede, C., et al. 2013. Biophys J. 104: 432-41. PMID: 23442865
  4. Measurement of in vitro microtubule polymerization by turbidity and fluorescence.  |  Mirigian, M., et al. 2013. Methods Cell Biol. 115: 215-29. PMID: 23973075
  5. Monitoring and modulating ion traffic in hybrid lipid/polymer vesicles.  |  Paxton, WF., et al. 2017. Colloids Surf B Biointerfaces. 159: 268-276. PMID: 28800466
  6. Hydrogen ion buffers for biological research.  |  Good, NE., et al. 1966. Biochemistry. 5: 467-77. PMID: 5942950
  7. Hyperosmolar triggering of histamine release from human basophils.  |  Findlay, SR., et al. 1981. J Clin Invest. 67: 1604-13. PMID: 6165738
  8. In vitro basophil histamine release induced by mannitol in a patient with a mannitol-induced anaphylactoid reaction.  |  Findlay, SR., et al. 1984. J Allergy Clin Immunol. 73: 578-83. PMID: 6201521
  9. Effects of NaOH-PIPES buffer used in aldehyde fixative on alkaline phosphatase activity in rat hepatocytes.  |  Yamamoto, K. and Ogawa, K. 1983. Histochemistry. 77: 339-51. PMID: 6305886
  10. Buffers for enzymes.  |  Blanchard, JS. 1984. Methods Enzymol. 104: 404-14. PMID: 6717292
  11. The imidazole-promoted inactivation of horse-liver alcohol dehydrogenase.  |  Dahl, KH. and McKinley-McKee, JS. 1981. Eur J Biochem. 120: 451-9. PMID: 7037391
  12. Nuclear-cytoplasmic translocations of protein p26 during aerobic-anoxic transitions in embryos of Artemia franciscana.  |  Clegg, JS., et al. 1995. Exp Cell Res. 219: 1-7. PMID: 7628526
  13. A C. elegans mutant screen based on antibody or histochemical staining.  |  Xie, G., et al. 1995. Genet Anal. 12: 95-100. PMID: 8574900
  14. Effects of electroporation on the tubulin cytoskeleton and directed migration of corneal fibroblasts cultured within collagen matrices.  |  Harkin, DG. and Hay, ED. 1996. Cell Motil Cytoskeleton. 35: 345-57. PMID: 8956005

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PIPES, Free Acid, 100 g

sc-216099
100 g
$107.00

PIPES, Free Acid, 1 kg

sc-216099A
1 kg
$576.00

PIPES, Free Acid, 5 kg

sc-216099B
5 kg
$1372.00

PIPES, Free Acid, 10 kg

sc-216099C
10 kg
$2602.00