PFKFB4 Double Nickase Plasmid (h): sc-410933-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PFKFB4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PFKFB4 Double Nickase Plasmid (h) and PFKFB4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PFKFB4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PFKFB4 Antibody (A-1): sc-514792
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PFKFB4 Double Nickase Plasmid (h) | sc-410933-NIC | 20 µg | $410.00 | |||
PFKFB4 Double Nickase Plasmid (h2) | sc-410933-NIC-2 | 20 µg | $410.00 |
PFKFB4 encodes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, a bifunctional enzyme that controls cellular levels of fructose-2,6-bisphosphate, a potent allosteric activator of PFK-1 and a key regulator of glycolytic flux. By tuning the balance between glycolysis and the pentose phosphate pathway, PFKFB4 supports bioenergetic homeostasis, redox control through NADPH production, and biosynthetic precursor availability. PFKFB4 activity links nutrient sensing to metabolic reprogramming and can influence proliferation, survival under stress, and responses to hypoxia. Dysregulated PFKFB4 expression or function has been associated with altered metabolic phenotypes observed in cancer and other disorders characterized by perturbed glucose metabolism.
PFKFB4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PFKFB4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PFKFB4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PFKFB4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PFKFB4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.