Pentafluorobenzenesulfonyl fluorescein (CAS 728912-45-6)

Pentafluorobenzenesulfonyl fluorescein is a chemically modified variant of fluorescein, widely recognized for its application in fluorescent labeling techniques in molecular biology and biochemical research. This compound leverages the intrinsic properties of fluorescein, known for its robust photostability and intense fluorescence under blue light excitation, but is modified to increase its reactivity and specificity towards target molecules. The pentafluorobenzenesulfonyl group enhances the molecule′s electrophilic character, facilitating its conjugation to various biomolecules, including proteins, nucleic acids, and lipids, through sulfonamide or ester linkages. This characteristic makes it an invaluable tool in the study of cellular processes, allowing for real-time visualization of dynamic biological interactions and pathways with high spatial and temporal resolution. The unique properties of pentafluorobenzenesulfonyl fluorescein make it particularly useful in applications such as fluorescence microscopy, flow cytometry, and fluorescence resonance energy transfer (FRET) experiments, where it contributes to a deeper understanding of cellular and molecular mechanics.