PDP2 CRISPR/Cas9 KO Plasmid (h): sc-406956
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDP2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PDP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDP2 CRISPR/Cas9 KO Plasmid (h) | sc-406956 | 20 µg | $397.00 |
Overview
Pyruvate dehydrogenase phosphatase catalytic subunit 2 (PDP2) is a mitochondrial enzyme that dephosphorylates and activates the pyruvate dehydrogenase complex, thereby promoting conversion of pyruvate to acetyl-CoA and coordinating glycolytic carbon entry into the TCA cycle. By tuning PDH activity, PDP2 contributes to metabolic flexibility, redox balance, and cellular energy homeostasis, linking nutrient availability to oxidative metabolism. Altered regulation of the PDH–PDP axis is associated with metabolic reprogramming observed in disorders affecting mitochondrial function and in contexts of abnormal glucose and lipid utilization, making PDP2 a useful node for studying mitochondrial bioenergetics. PDP2 also provides a handle to interrogate signaling inputs that modulate PDH phosphorylation state and downstream acetyl-CoA-dependent processes.
PDP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PDP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PDP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PDP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PDP2 protein expression.
This CRISPR knockout system enables efficient generation of PDP2-deficient cell models for investigation of PDP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PDP2 exon(s) critical for PDP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PDP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PDP2 CRISPR/Cas9 KO Plasmid (h) and PDP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PDP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PDP2 HDR Plasmid (h) and PDP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PDP2 homology arms to support homology-directed repair at defined PDP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.