PCID2 CRISPR/Cas9 KO Plasmid (h): sc-406324
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PCID2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PCID2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCID2 CRISPR/Cas9 KO Plasmid (h) | sc-406324 | 20 µg | $397.00 |
Overview
PCID2 (PCI domain containing 2) encodes a PCI-domain protein implicated in the assembly and regulation of large multiprotein complexes that coordinate RNA metabolism and gene expression control. PCID2 has been linked to post-transcriptional regulatory processes, including mRNA surveillance and turnover, and is studied in the context of proteostasis-associated pathways that shape cellular homeostasis. Perturbation of these processes can influence cell-cycle progression, stress responses, and differentiation programs by altering transcript stability and protein output. Dysregulated RNA processing and quality-control networks are frequently associated with oncogenic states and other complex diseases, making PCID2 a useful target for mechanistic studies of gene regulatory circuitry.
PCID2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PCID2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PCID2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PCID2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PCID2 protein expression.
This CRISPR knockout system enables efficient generation of PCID2-deficient cell models for investigation of PCID2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PCID2 exon(s) critical for PCID2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PCID2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PCID2 CRISPR/Cas9 KO Plasmid (h) and PCID2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PCID2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PCID2 HDR Plasmid (h) and PCID2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PCID2 homology arms to support homology-directed repair at defined PCID2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.