Pcdhb3 CRISPR/Cas9 KO Plasmid (m): sc-430065
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pcdhb3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pcdhb3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pcdhb3 CRISPR/Cas9 KO Plasmid (m) | sc-430065 | 20 µg | $397.00 |
Overview
Pcdhb3 encodes protocadherin beta 3, a member of the clustered protocadherin family implicated in calcium-dependent cell–cell adhesion and the establishment of neuronal identity. In the nervous system, protocadherins contribute to synapse formation, neurite outgrowth, and self-avoidance mechanisms that refine neural circuit connectivity. Pcdhb3 function is linked to membrane adhesion complexes and downstream cytoskeletal organization processes that shape dendritic and axonal patterning. Dysregulation of protocadherin gene clusters has been associated with altered neurodevelopmental trajectories and synaptic dysfunction, supporting investigation of Pcdhb3 in models of circuit assembly and neurological phenotypes.
Pcdhb3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pcdhb3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pcdhb3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pcdhb3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pcdhb3 protein expression.
This CRISPR knockout system enables efficient generation of Pcdhb3-deficient cell models for investigation of Pcdhb3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pcdhb3 exon(s) critical for Pcdhb3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pcdhb3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pcdhb3 CRISPR/Cas9 KO Plasmid (m) and Pcdhb3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pcdhb3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pcdhb3 HDR Plasmid (m) and Pcdhb3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pcdhb3 homology arms to support homology-directed repair at defined Pcdhb3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.