Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p57 Kip2 Double Nickase Plasmid (h): sc-400444-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p57 Kip2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- p57 Kip2 Double Nickase Plasmid (h) and p57 Kip2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDKN1C. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p57 Kip2 Antibody (KP39): sc-56341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p57 Kip2 Double Nickase Plasmid (h) | sc-400444-NIC | 20 µg | $410.00 | |||
p57 Kip2 Double Nickase Plasmid (h2) | sc-400444-NIC-2 | 20 µg | $410.00 |
CDKN1C encodes the cyclin-dependent kinase inhibitor p57 Kip2, a key negative regulator of G1/S progression that restrains CDK activity to enforce cell-cycle exit and support differentiation. As an imprinted gene predominantly expressed from the maternal allele, CDKN1C helps coordinate developmental growth programs and tissue homeostasis, intersecting with pathways governing proliferation, apoptosis, and lineage commitment. Altered CDKN1C expression or function is associated with dysregulated growth control and has been implicated in developmental overgrowth phenotypes and tumor biology, making it a useful node for studying cell-cycle checkpoints. In human cells, p57 Kip2 is also linked to senescence-like states and context-dependent responses to mitogenic signaling and stress.
p57 Kip2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDKN1C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDKN1C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDKN1C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDKN1C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.