p-MBP Antibody (D-11): sc-365621

p-MBP Antibody (D-11) is a mouse monoclonal IgG3 kappa light chain antibody that detects p-MBP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-p-MBP antibody (D-11) is available as a non-conjugated format. Myelin basic protein (MBP) serves as a crucial component of the myelin sheath in the central nervous system, playing a vital role in axon insulation and efficient electrical signal transmission. p-MBP phosphorylation at Threonine 125 is essential for regulating myelin sheath stability, which maintains proper neuronal function. This phosphorylation process is influenced by mitogen-activated protein kinases during myelinogenesis and in conditions such as multiple sclerosis. MBP phosphorylation is modulated by high-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus, highlighting p-MBP′s importance in response to neuronal activity. During heightened neuronal signaling, calcium influx activates axonal nitric oxide synthase, leading to nitric oxide and superoxide production, which regulate MBP phosphorylation through MAPK pathways. Understanding these mechanisms is vital for elucidating demyelinating disease pathophysiology and developing potential therapeutic strategies.

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p-MBP Antibody (D-11) References:

1. Regulation of myelin basic protein phosphorylation by mitogen-activated protein kinase during increased action potential firing in the hippocampus.  |  Atkins, CM., et al. 1999. J Neurochem. 73: 1090-7. PMID: 10461899
2. Knockdown of MBP-1 in human foreskin fibroblasts induces p53-p21 dependent senescence.  |  Ghosh, AK., et al. 2008. PLoS One. 3: e3384. PMID: 18852884
3. Hypoxia induces differential translation of enolase/MBP-1.  |  Sedoris, KC., et al. 2010. BMC Cancer. 10: 157. PMID: 20412594
4. Structure and function of the proline-rich region of myelin basic protein.  |  Fraser, PE. and Deber, CM. 1985. Biochemistry. 24: 4593-8. PMID: 2415158
5. Identification of an antigenic determinant within the phylogenetically conserved triprolyl region of myelin basic protein.  |  Potter, NT., et al. 1986. J Immunol. 136: 516-20. PMID: 2416809
6. The glycosylation of human myelin basic protein at threonines 95 and 98 occurs sequentially.  |  Persaud, R., et al. 1988. Biochim Biophys Acta. 966: 357-61. PMID: 2458139
7. Nuclear Enolase-1/ MBP-1 expression and its association with the Wnt signaling in epithelial ovarian cancer.  |  Czogalla, B., et al. 2021. Transl Oncol. 14: 100910. PMID: 33069100
8. Generation and Release of Neurogranin, Vimentin, and MBP Proteolytic Peptides, Following Traumatic Brain Injury.  |  Sarkis, GA., et al. 2022. Mol Neurobiol. 59: 731-747. PMID: 34762230
9. MBP-11901 Inhibits Tumor Growth of Hepatocellular Carcinoma through Multitargeted Inhibition of Receptor Tyrosine Kinases.  |  Park, HJ., et al. 2022. Cancers (Basel). 14: PMID: 35454900
10. Identification of a mitogen-activated protein kinase site in human myelin basic protein in situ.  |  Yon, M., et al. 1996. J Neuroimmunol. 65: 55-9. PMID: 8642064

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-MBP Antibody (D-11) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-p-MBP Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-MBP Antibody (D-11) Specifications

Alternate Product Name: myelin basic protein

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: Thr 125

Epitope Target: MBP

Epitope Species: human

Product Reactivity/Detection: Thr 125 phosphorylated MBP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for p-MBP Antibody (D-11): P: Blocking Peptide

The preferred MBP antibody is MBP Antibody (F-6): sc-271524.