p-Akt1/2/3 Antibody (B-12): sc-377556

p-Akt1/2/3 Antibody (B-12) is a mouse monoclonal IgM antibody that detects p-Akt1/2/3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Akt1/2/3 monoclonal antibody (B-12) is available in a non-conjugated form, allowing for versatile experimental use. The Akt family of serine/threonine kinases, which includes Akt1, Akt2, and Akt3, plays a crucial role in various cellular processes, including metabolism, cell survival, and growth. Notably, Akt proteins are activated by growth factors such as insulin and insulin-like growth factor 1 (IGF-1), leading to their phosphorylation at specific threonine and serine residues. This phosphorylation enables Akt to transduce signals from the phosphatidylinositol 3-kinase (PI3K) pathway, which regulates cellular responses to growth signals. The interaction of Akt with other proteins, such as phosphoinositide-dependent kinase 1 (PDK1), is critical for activation and subsequent signaling cascade. Akt3, expressed in tissues like adipocytes and skeletal muscle, is involved in processes such as glucose uptake and glycogen synthesis, highlighting importance in metabolic regulation. p-Akt1/2/3 (B-12) antibody specifically recognizes phosphorylated forms of Akt1, Akt2, and Akt3, making this antibody an invaluable tool for researchers studying signaling pathways that govern cell growth and metabolism.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Akt1/2/3 Antibody (B-12) has 38 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Akt1/2/3 Antibody (B-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Akt1/2/3 Antibody (B-12) Specifications

Alternate Product Name: Akt1/2/3

Product Clone Name: B-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Thr 450

Epitope Target: Akt1

Epitope Species: human

Product Reactivity/Detection: Thr 450 phosphorylated Akt1 and Thr 451 correspondingly phosphorylated Akt2 and Thr 447 correspondingly phosphorylated Akt3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 38 citations in various scientific publications

Available Conjugates/Forms for p-Akt1/2/3 Antibody (B-12): P: Blocking Peptide

The preferred Akt1 antibody is Akt1 Antibody (B-1): sc-5298.