OTUD6B Double Nickase Plasmid (h): sc-407492-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTUD6B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OTUD6B Double Nickase Plasmid (h) and OTUD6B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OTUD6B. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUD6B Double Nickase Plasmid (h) | sc-407492-NIC | 20 µg | $410.00 | |||
OTUD6B Double Nickase Plasmid (h2) | sc-407492-NIC-2 | 20 µg | $410.00 |
OTUD6B encodes an OTU family deubiquitinating enzyme that hydrolyzes ubiquitin conjugates to shape protein stability and signaling amplitude. By reversing ubiquitination, OTUD6B contributes to proteostasis and modulation of ubiquitin-dependent pathways, including stress responses and immune-related signaling cascades. Altered OTUD6B activity has been associated with dysregulated cellular homeostasis and has been investigated in the context of neurodevelopmental and immunological phenotypes, supporting its relevance for mechanistic studies of ubiquitin system control. Human OTUD6B is therefore a useful node for dissecting how deubiquitination influences pathway dynamics and cell state decisions.
OTUD6B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OTUD6B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OTUD6B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OTUD6B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OTUD6B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.