Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Orexin R-1 CRISPR Activation Plasmid (h): sc-401613-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Orexin R-1 CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Orexin R-1 CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Orexin R-1 CRISPR Activation Plasmid (h) and Orexin R-1 CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the HCRTR1 transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Orexin R-1 CRISPR Activation Plasmid (h) | sc-401613-ACT | 20 µg | $397.00 |
HCRTR1 encodes orexin receptor 1 (Orexin R-1), a class A GPCR that binds the neuropeptides orexin-A and orexin-B to regulate arousal, sleep–wake stability, feeding behavior, reward processing, and neuroendocrine homeostasis. Upon ligand engagement, Orexin R-1 couples to Gq/11 and other G proteins to activate PLCβ, elevate intracellular Ca2+, and stimulate downstream MAPK/ERK signaling, with additional cross-talk to cAMP/PKA and PI3K pathways depending on cell context. This signaling influences neuronal excitability and synaptic plasticity, integrating hypothalamic inputs with broader brain circuits. Dysregulated orexin signaling and HCRTR1 activity have been linked to sleep and circadian phenotypes and are frequently studied in relation to stress responsivity, metabolic control, and neuropsychiatric disease-associated pathways.
Orexin R-1 CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous HCRTR1 expression without altering the underlying DNA sequence.
Orexin R-1 CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the HCRTR1 locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the HCRTR1 transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous Orexin R-1 expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native HCRTR1 locus and enabling the study of Orexin R-1-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of Orexin R-1 pathway restoration in tumor cells with silenced or reduced HCRTR1 expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.