OBSL1 Double Nickase Plasmid (h): sc-407284-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OBSL1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OBSL1 Double Nickase Plasmid (h) and OBSL1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OBSL1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OBSL1 Double Nickase Plasmid (h) | sc-407284-NIC | 20 µg | $410.00 | |||
OBSL1 Double Nickase Plasmid (h2) | sc-407284-NIC-2 | 20 µg | $410.00 |
OBSL1 (obscurin-like protein 1) encodes a large cytoskeletal adaptor that localizes to actin-associated structures and contributes to cell architecture, adhesion, and mechanotransduction. Through scaffold-like interactions, OBSL1 supports organization of protein complexes that influence cytoskeletal dynamics, intracellular trafficking, and signaling at the cell cortex. Loss-of-function variants in OBSL1 are associated with 3M syndrome, a growth disorder that highlights the gene’s role in cellular proliferation and structural integrity. In research settings, OBSL1 is studied in the context of cytoskeleton-regulated pathways, extracellular matrix coupling, and stress-responsive signaling that shape tissue development and cell morphology.
OBSL1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OBSL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OBSL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OBSL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OBSL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.