Nurr1 Double Nickase Plasmid (h): sc-400289-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nurr1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nurr1 Double Nickase Plasmid (h) and Nurr1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR4A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nurr1 Antibody (F-5): sc-376984
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nurr1 Double Nickase Plasmid (h) | sc-400289-NIC | 20 µg | $410.00 | |||
Nurr1 Double Nickase Plasmid (h2) | sc-400289-NIC-2 | 20 µg | $410.00 |
NR4A2 encodes Nurr1, an orphan nuclear receptor transcription factor that regulates gene programs involved in neuronal differentiation, mitochondrial homeostasis, and cellular stress responses. Nurr1 coordinates transcriptional networks with other nuclear receptors and co-regulators to modulate dopamine-related signaling, inflammatory gene expression, and survival pathways. Altered NR4A2 activity has been linked to dysregulated neuroimmune signaling and impaired maintenance of dopaminergic phenotypes, making it a relevant target for studying neurodegeneration and inflammation-associated neuronal dysfunction. In human cell models, NR4A2 perturbation supports mechanistic investigation of transcriptional control in neural development and stress-adaptive responses.
Nurr1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR4A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR4A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR4A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR4A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.