NSUN2 Double Nickase Plasmid (h): sc-405097-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NSUN2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NSUN2 Double Nickase Plasmid (h) and NSUN2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NSUN2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NSUN2 Double Nickase Plasmid (h) | sc-405097-NIC | 20 µg | $410.00 | |||
NSUN2 Double Nickase Plasmid (h2) | sc-405097-NIC-2 | 20 µg | $410.00 |
NSUN2 encodes an RNA cytosine-5 methyltransferase that deposits m5C modifications on diverse RNA substrates, including tRNAs and mRNAs, influencing RNA stability, translation efficiency, and stress responses. By regulating tRNA methylation and RNA processing, NSUN2 contributes to ribosome biogenesis, cell-cycle progression, and coordinated proteostasis programs. Altered NSUN2 activity has been associated with disrupted RNA modification landscapes and downstream changes in gene expression, linking it to phenotypes involving impaired growth control and cellular stress adaptation. These features make NSUN2 a useful entry point for studying epitranscriptomic regulation and post-transcriptional control mechanisms in human cells.
NSUN2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NSUN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NSUN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NSUN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NSUN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.