NOTCH2NL Double Nickase Plasmid (h): sc-417192-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NOTCH2NL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NOTCH2NL Double Nickase Plasmid (h) and NOTCH2NL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOTCH2NL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NOTCH2NL Antibody (39-Y): sc-100307
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NOTCH2NL Double Nickase Plasmid (h) | sc-417192-NIC | 20 µg | $410.00 | |||
NOTCH2NL Double Nickase Plasmid (h2) | sc-417192-NIC-2 | 20 µg | $410.00 |
NOTCH2NL encodes a human-specific Notch2-like protein family member that modulates canonical Notch signaling, a pathway central to cell fate specification, proliferation, and differentiation during neurodevelopment. By influencing Notch receptor activity and downstream transcriptional programs, NOTCH2NL contributes to regulation of neural progenitor maintenance and timing of neuronal differentiation. Copy number variation and altered expression of NOTCH2NL paralogs have been associated with human brain development phenotypes and neurodevelopmental disorders, supporting its relevance for studies of cortical expansion, synaptic maturation, and developmental gene regulatory networks.
NOTCH2NL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOTCH2NL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOTCH2NL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOTCH2NL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOTCH2NL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.