Nopp140 Antibody (3B4): sc-101101
- Nopp140 Antibody (3B4) is a mouse monoclonal IgG3 κ provided at 100 µg/ml
- raised against recombinant Nopp140 of human origin
- recommended for detection of Nopp140 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nopp140 Antibody (3B4). This work is in progress.
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Nopp140 Antibody (3B4) is a mouse monoclonal IgG3 antibody that detects Nopp140 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Nopp140 is a crucial nucleolar phosphoprotein that plays a significant role in cellular processes by functioning as both a chaperone for the import and export of proteins from the nucleolus and as a transcription factor. Nopp140 was initially identified in rat liver as a nuclear localization signal-binding protein and has since been characterized as an interacting partner of RNA Polymerase, which is essential for the transcription of DNA into RNA. Nopp140′s ability to associate with the general transcription factor TFIIB and the protein kinase casein kinase II (CKII) highlights its importance in the regulation of gene expression, as CKII-mediated phosphorylation of Nopp140 enhances its binding to nuclear localization signals. Furthermore, Nopp140′s colocalization with another nucleolar protein, NAP57, in the nucleolus and coiled bodies suggests its involvement in the intricate activities that occur within these cellular structures, making Nopp140 a vital component in the orchestration of ribosome biogenesis and RNA processing.
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Nopp140 Antibody (3B4) References:
- Anisoosmotic regulation of the Nopp140 mRNA in H4IIE rat hepatoma cells and primary hepatocytes. | Warskulat, U., et al. 1999. FEBS Lett. 452: 259-62. PMID: 10386602
- Human Nopp140, which interacts with RNA polymerase I: implications for rRNA gene transcription and nucleolar structural organization. | Chen, HK., et al. 1999. Mol Cell Biol. 19: 8536-46. PMID: 10567578
- Intranuclear endoplasmic reticulum induced by Nopp140 mimics the nucleolar channel system of human endometrium. | Isaac, C., et al. 2001. J Cell Sci. 114: 4253-64. PMID: 11739657
- Nopp140 shuttles on tracks between nucleolus and cytoplasm. | Meier, UT. and Blobel, G. 1992. Cell. 70: 127-38. PMID: 1623516
- Characterization of the InsP6-dependent interaction between CK2 and Nopp140. | Lee, WK., et al. 2008. Biochem Biophys Res Commun. 376: 439-44. PMID: 18790693
- Three-Dimensional Distribution of UBF and Nopp140 in Relationship to Ribosomal DNA Transcription During Mouse Preimplantation Development. | Koné, MC., et al. 2016. Biol Reprod. 94: 95. PMID: 26984997
- Nopp140-mediated concentration of telomerase in Cajal bodies regulates telomere length. | Bizarro, J., et al. 2019. Mol Biol Cell. 30: 3136-3150. PMID: 31664887
- Nopp140-chaperoned 2'-O-methylation of small nuclear RNAs in Cajal bodies ensures splicing fidelity. | Bizarro, J., et al. 2021. Genes Dev. 35: 1123-1141. PMID: 34301768
- Specific interaction between casein kinase 2 and the nucleolar protein Nopp140. | Li, D., et al. 1997. J Biol Chem. 272: 3773-9. PMID: 9013635
- Nopp140 functions as a molecular link between the nucleolus and the coiled bodies. | Isaac, C., et al. 1998. J Cell Biol. 142: 319-29. PMID: 9679133
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nopp140 Antibody (3B4) | sc-101101 | 100 µg/ml | $339.00 |