Nop25 Double Nickase Plasmid (h): sc-408434-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nop25 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nop25 Double Nickase Plasmid (h) and Nop25 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOL12. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nop25 Antibody (E-2): sc-374257
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nop25 Double Nickase Plasmid (h) | sc-408434-NIC | 20 µg | $410.00 | |||
Nop25 Double Nickase Plasmid (h2) | sc-408434-NIC-2 | 20 µg | $410.00 |
Human NOL12 encodes the nucleolar protein Nop25, a conserved factor implicated in RNA metabolism and nucleolar homeostasis. Nop25 has been associated with ribosomal RNA processing and maturation, linking it to ribosome biogenesis and the coordination of nucleolar stress responses. Through these processes, NOL12 can influence global translational capacity and cell-cycle–coupled growth programs that depend on efficient rRNA production. Altered nucleolar function and dysregulated RNA processing pathways involving NOL12 are frequently studied in the context of proliferative disorders and genome maintenance phenotypes in model systems.
Nop25 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOL12 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOL12. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOL12 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOL12-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.