Nε-DNP-L-lysine hydrochloride (CAS 14401-10-6)

Nε-DNP-L-lysine hydrochloride, known as DNPL, is a synthetic compound extensively utilized in scientific research for its remarkable ability to interact with proteins and other molecules. As a variant of lysine, an essential amino acid, Nε-DNP-L-lysine hydrochloride belongs to the 2,4-dinitrophenyl family of compounds. Its versatility has led to its application in a wide array of research applications. Nε-DNP-L-lysine hydrochloride′s prominent role in scientific research arises from its capacity to bind with proteins and other molecules. It finds extensive use in various biochemical studies, such as investigations into protein-protein interactions, enzyme kinetics, and receptor-ligand interactions. Additionally, Nε-DNP-L-lysine hydrochloride has proven valuable in laboratory experiments for quantifying the binding affinity of proteins and other molecules. The binding mechanism of Nε-DNP-L-lysine hydrochloride involves the formation of a covalent bond between the 2,4-dinitrophenyl group of DNPL and the amine group present in the protein or molecule. Notably, this binding is reversible, and it can be disrupted by elevating the pH level or introducing a reducing agent.